MfMel1基因参与调控桃褐腐病菌对桃果实的致病力  

MfMel1 gene participates in the pathogenicity of pathogen fungus Monilinia fructicola on peach fruits

作　　者：谷文倩 阴伟晓 罗朝喜[1,2] Gu Wenqian;Yin Weixiao;Luo Chaoxi(National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops,Huazhong Agricultural University,Wuhan 430070,Hubei Province,China;Hubei Key Laboratory of Plant Pathology,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,Hubei Province,China)

机构地区：[1]华中农业大学,果蔬园艺作物种质创新与利用全国重点实验室,武汉430070 [2]华中农业大学植物科技学院,湖北省作物病害监测及安全控制重点实验室,武汉430070

出　　处：《植物保护学报》2023年第2期380-390,共11页Journal of Plant Protection

基　　金：财政部和农业农村部国家现代农业产业技术体系(CARS-30)。

摘　　要：为明确MfMel1基因在桃褐腐病菌Monilinia fructicola中的功能,利用生物信息学软件对该基因的结构、所编码蛋白的保守结构域以及进化关系进行分析;通过遗传转化方法对该基因进行敲除,并进行表型分析。结果显示,敲除转化子△MfMel1菌株的菌丝生长速率、孢子萌发率与野生型菌株Bmpc7均无显著差异,但产孢量显著降低。△MfMel1菌株在0.01%SDS、600μg/mL刚果红、150 g/L甘油、200 g/L蔗糖、6 mmol/L H_(2)O_(2)、4%NaCl和1.2 mmol/L山梨醇培养基上的生长速率、菌丝形态均无显著变化,但对200 g/L葡萄糖的渗透胁迫敏感性增强。敲除转化子△MfMel1菌株的α-半乳糖苷酶活性明显降低,对桃果实的致病力显著下降。表明MfMel1基因在桃褐腐病菌中可能通过参与调控α-半乳糖苷酶酶活,从而参与病原菌的致病。In order to illustrate the functions of MfMel1 in pathogen fungus Monilinia fructicola,the gene structure,conserved domain and evolutionary relationships of the predicted protein were analyzed using bioinformatic software.The gene was knocked out through the genetic transformation,and the phenotypes of the mutants were investigated.No significant difference was observed for the mycelial growth and spore germination between knockout transformants△MfMel1 and wild type strain Bmpc7.However,the sporulation of△MfMel1 strains decreased significantly compared to Bmpc7.△MfMel1 strains showed increased sensitivity to 200 g/L glucose,but not to 0.01%SDS,600μg/mL congo red,150g/Lglycerol,200g/Lsucrose,6mmol/LH_(2)O_(2),4%NaCl,or1.2mmol/Lsorbitol.△MfMel1 strains exhibited significantly decreased virulence on peach fruit and reduced activity ofα-galactosidase.The results indicated that MfMel1 was involved in regulating theα-galactosidase activity,thus playing an important role during the infection of M.fructicola on peach fruit.

关 键 词：桃褐腐病菌 MfMel1基因 糖苷水解酶 侵染机制 Α-半乳糖苷酶 

分 类 号：S436.621.13[农业科学—农业昆虫与害虫防治]