草地贪夜蛾对国产Bt玉米的抗性风险评估  

A risk assessment of the resistance of fall armyworm Spodoptera frugiperda to Bt maize in China

作　　者：王文荟 黄运新[1] 万鹏[2] 封洪强[3] 吴孔明[4] Wang Wenhui;Huang Yunxin;Wan Peng;Feng Hongqiang;Wu Kongming(School of Resource and Environmental Science,Hubei University,Wuhan 430062,Hubei Province,China;Institute of Plant Protection and Soil Fertility,Hubei Academy of Agricultural Sciences,Wuhan 430064,Hubei Province,China;Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan Province,China;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]湖北大学资源环境学院,武汉430062 [2]湖北省农业科学院植物保护土肥研究所,武汉430064 [3]河南省农业科学院植物保护研究所,郑州450002 [4]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《植物保护学报》2023年第2期413-420,共8页Journal of Plant Protection

基　　金：国家自然科学基金(31670543);国家转基因生物新品种培育重大专项(2019ZX08012-004)。

摘　　要：为评估草地贪夜蛾Spodoptera frugiperda对国产Bt玉米的抗性风险,基于抗性风险分析模型对草地贪夜蛾在3种Bt玉米和2种庇护所条件下的抗性时间进行预测分析。结果显示,无论是采用种子混合庇护所还是结构化庇护所,草地贪夜蛾对Cry1Ab+Vip3Aa-玉米的抗性时间均远长于对Vip3Aa-玉米和Cry1Ab-玉米的抗性时间。在Bt与非Bt植株之间存在异花授粉和幼虫转移的条件下,采用种子混合庇护所的抗性时间较采用结构化庇护所的抗性时间大大缩短。以采用Cry1Ab+Vip3Aa-玉米和比例为0.20的庇护所为例,当异花授粉Bt显性度为0.5、幼虫转移概率为0.95时,采用结构化庇护所的抗性时间超过200代,而采用种子混合庇护所的抗性时间只有59代。结果表明,草地贪夜蛾对Cry1Ab-玉米的抗性风险远大于对Cry1Ab+Vip3Aa-玉米的抗性风险,而种子混合庇护所条件下的抗性风险远大于结构化庇护所条件下的抗性风险。In order to understand the resistance risk of the fall armyworm,Spodoptera frugiperda,to Bt maize in China,the time for developing resistance was predicted by using the resistance risk analysis model for three Bt maize varieties and two types of refuges.The results showed that,for both seed mix refuge or structured refuge,the time for developing resistance was much longer for Cry1Ab+Vip3Aatransgenic maize than for Cry1Ab-transgenic maize or Vip3Aa-transgenic maize.In the case of crosspollination and larval movement between Bt and non-Bt maize plants,the time for developing resistance was generally shorter for seed mix refuge than for structured refuge.When the Bt dominance of cross-pollination was 0.5,the probability of larval movement was 0.95,if the proportion of refuge was 0.20,the time for developing resistance for Cry1Ab+Vip3Aa-transgenic maize was longer than 200 generations for structured refuge,but only 59 generations for seed mix refuge.These results suggested that the resistance risk of S.frugiperda to Cry1Ab-maize was much higher than to Cry1Ab+Vip3Aa-maize,and the resistance risk in the case of seed mix refuge was much higher than in the case of structured refuge.

关 键 词：草地贪夜蛾 BT玉米 种子混合庇护所 幼虫转移 抗性风险分析 

分 类 号：S435.132[农业科学—农业昆虫与害虫防治]