香烟烟雾提取物对人支气管上皮细胞中脂质代谢的影响研究  

Effects of cigarette smoke extracts on lipid metabolism in human bronchial epithelial cells

作　　者：张亚萍 王礼兴[1] 颜芙蓉 ZHANG Yaping;WANG Lixing;YAN Furong(Central Laboratory,the Second Affiliated Hospital of Fujian Medical University,Quanzhou,Fujian 362000,China)

机构地区：[1]福建医科大学附属第二医院中心实验室,福建泉州362000

出　　处：《现代医药卫生》2023年第9期1441-1445,1450,共6页Journal of Modern Medicine & Health

基　　金：福建省自然科学基金项目(2019J01171);福建省卫生健康委员会青年科研课题(2018-2-25);福建省泉州市科学技术局科技计划项目(2018Z122;2018N008S)。

摘　　要：目的基于液相色谱-质谱联用(LC-MS)技术研究香烟烟雾提取物(CSE)对人支气管上皮细胞(HBECs)中脂质代谢的影响。方法体外建立CSE干预HBECs模型,以5%CSE干预的支气管上皮细胞为实验组,以无CSE干预的细胞为对照组,采用LC-MS技术定量检测HBECs内的脂质成分,数据经主成分分析及正交偏最小二乘法判别分析,从728个脂质(11类脂质)中筛选出差异脂质。结果与对照组比较,实验组的HBECs显示出191个差异表达脂质,有185个脂质上调,6个脂质下调,其中增高数量比例最高的脂质为鞘脂途径脂质,增高倍数最大的脂质为甘油三酯。结论CSE可导致HBECs的脂质代谢紊乱,尤其是鞘脂途径脂质的积累,可能与慢性阻塞性肺疾病进程相关。Objective To investigate the effects of cigarette smoke extracts(CSE)on lipid metabolism in human bronchial epithelial cells(HBECs)based on liquid chromatography-mass spectrometry(LC-MS)technology.Methods A cell model of HBECs treated with CSE was established in vitro,with 5%CSE intervention bronchial epithelial cells as the experimental group and no CSE intervention cells as the control group.LC-MS was used to quantitatively detect lipids in HBECs.The data was analyzed by PCA analysis and OPLS-DA analysis,the differential lipids were screened out from 728 lipids(11 categories).Results Compared with the control group,CSE-induced HBECs showed 191 differentially expressed lipids,of which 185 lipids were up-regulated and six lipids were down-regulated.Among them,the lipids with the highest proportion of increased numbers were sphingolipid pathway lipids,and with the highest increase ratio was triglycerides.Conclusion CSE causing the lipid metabolism disorders in HBECs,especially the accumulation of lipids in the sphingolipid pathway,may be associated with the chronic obstructive pulmonary disease process.

关 键 词：脂质组学 液相色谱-质谱联用 香烟烟雾提取物 人支气管上皮细胞 鞘脂 

分 类 号：Q591.5[生物学—生物化学]