元江普通野生稻中抗白叶枯病基因鉴定与分析  

Identification and Analysis of Resistance Genes to Bacterial Blight in Yuanjiang Common Wild Rice

作　　者：卢源达 钟巧芳[1] 王波[1] 张敦宇[1] 殷富有[1] 王玲仙[1] 程在全[1] 陈玲[1] LU Yuanda;ZHONG Qiaofang;WANG Bo;ZHANG Dunyu;YIN Fuyou;WANG Lingxian;CHENG Zaiquan;CHEN Ling(Biotechnology and Germplasm Resources Institute,Yunnan Academy of Agricultural Sciences,Yunnan Provincial Key Laboratory of Agricultural Biotechnology,Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation,Ministry of Agriculture,Kunming 650205,China;College of Plant Protection,Yunnan Agricultural University,Kunming 650224,China)

机构地区：[1]云南省农业科学院生物技术与种质资源研究所,云南省农业生物技术重点实验室,农业部西南作物基因资源与种质创制重点实验室,云南昆明650205 [2]云南农业大学植物保护学院,云南昆明650224

出　　处：《华北农学报》2023年第2期199-205,共7页Acta Agriculturae Boreali-Sinica

基　　金：云南省颜龙安院士专家工作站(202005AF150032);云南省基础研究专项面上项目(202001AT070015,202001AT070003);云南省科技人才和平台计划项目(2019HB034,202005AM070029);云南省青年拔尖人才专项(YNWR-QNBJ-2018-284);国家自然科学基金地区项目(31960374);中央引导地方科技发展资金计划(202207AB110012);云南种子种业联合实验室项目(202205AR070001)。

摘　　要：元江普通野生稻(YP)是一份被称为植物活化石的种质材料,同时其也拥有大量的抗性(R)基因。为了鉴定元江普通野生稻中含有目前已知白叶枯病抗性基因的情况。以元江普通野生稻(YP)、渗入系后代(L214和G252)及其渗入系的感病亲本栽培稻合系35(HX35)作为供试材料,通过接菌鉴定、功能标记检测、同源克隆和实时荧光定量(qRT-PCR)技术来评价这些材料对白叶枯病的抗性。接菌鉴定结果显示,YP、L214、G252对供试的白叶枯病菌C1、C2、C3、C4、C5、C6、C7、C9和PXO99^(A)均表现出抗性且病斑长度均小于2 cm;而HX35对供试的9个菌株均表现出感病且病斑长度远超6 cm。白叶枯病抗性基因检测结果显示,YP中含有Xa10的同源基因,HX35、L214、G252中含有xa23的感病同源基因。序列比对结果显示,抗病基因Xa10与YP中的Xa10启动子的效应因子结合元件(EBE_(AvrXa10))区域序列差异较大。同样地,隐性感病基因xa23与HX35、L214和G252中的xa23启动子的效应因子结合元件(EBE_(AvrXa23))区域序列一致。qRT-PCR结果显示,在接了PXO99^(A)菌株24 h后,YP、HX35、L214和G252中的免疫反应被激活,而YP中的Xa10和HX35、L214、G252中的xa23在所有处理时间段内均未被诱导表达。由此推测,YP、L214和G252中可能含有新的抗白叶枯病基因。Yuanjiang common wild rice(YP)is a germplasm material known as a living fossil in plants,which also has a large number of resistance(R)genes.Therefore,knowing the R genes in YP will help to apply different R genes to cultivated rice.Here,we identified the bacterial blight(BB)R genes in Yuanjiang common wild rice(YP),progeny of introgression lines(L214 and G252)and its susceptible parent,cultivated rice line 35(HX35).Meanwhile,the resistance of YP and its introgression lines to several Xanthomonas oryzae pv.oryzae(Xoo)strains was determined at the late tillering stage of rice.Under indoor control conditions,YP and its introgression lines showed resistance to 9 different Xoo strains(C1,C2,C3,C4,C5,C6,C7,C9 and PXO99^(A)),indicating that they had broad-spectrum resistance to BB.The results of bacterial blight resistance gene detection showed that YP contained homologous genes of Xa10,while HX35,L214 and G252 contained susceptible homologous genes of xa23.The sequence alignment results showed that the sequence of the effector binding element(EBE_(AvrXa10))region of the disease resistance gene Xa10 was significantly different from that of the Xa10 promoter in YP.Similarly,the recessive susceptible gene xa23 was consistent with the effector binding element(EBE_(AvrXa23))region of the xa23 promoter in HX35,L214 and G252.The qRT-PCR results showed that the immune responses in YP,HX35,L214 and G252 were activated after inoculation with strain PXO99^(A),but Xa10 in YP and xa23 in HX35,L214 and G252 were not induced to be expressed.It is thus hypothesized that YP,L214 and G252 may contain new genes for resistance to bacterial blight.

关 键 词：元江普通野生稻 渗入系 白叶枯病 抗性基因 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]