新型聚合物纳米粒子的制备及在铜离子和半胱氨酸检测中的应用  

Preparation and application of novel polymer nanoparticles in the detection of copper ion and cysteine

作　　者：张岩 赵松 谭胡静 向丹 ZHANG Yan;ZHAO Song;TAN Hujing;XIANG Dan(Chongqing Key Laboratory of Green Synthesis and Ap-plications,College of Chemistry,Chongqing Normal University,Chongqing 401331,China)

机构地区：[1]绿色合成与应用重庆市重点实验室,重庆师范大学化学学院,重庆401331

出　　处：《分析试验室》2023年第4期436-441,共6页Chinese Journal of Analysis Laboratory

基　　金：重庆市教委科技项目(KJQN202100502)资助。

摘　　要：基于二乙烯基蒽结构设计合成了一种两亲性共聚物,二(丙烯氧基苯乙烯)基蒽-丙烯酸共聚物(BASA-AA)。该聚合物能在水中快速自组装形成尺寸均一(45 nm)、不存在染料泄漏且高亮度(Φ=36%)的聚合物纳米粒子(BASA-AA NPs)。由于粒子表面分布有大量的羧基,能够稳定的分散在水中,并可用于纯水中Cu2+和半胱氨酸(Cys)的快速检测。该纳米粒子的荧光强度会在引入Cu2+后大幅降低,可实现对Cu2+的检测,检出限为45 nmol/L。得益于巯基与Cu2+之间较强的结合能力,可利用BASA-AA NPs与Cu2+组成的复合探针在0.1~10μmol/L范围内实现对Cys的检测,检出限为84 nmol/L。该方法的材料合成制备简单快速,检测速度快,在纯水中具有较高的选择性和灵敏度。A novel amphiphilic copolymer,dipropenoxystyrene anthracene and acrylic acid copolymer(BASA-AA)was designed and synthesized based on divinyl anthracene.The polymer self-assembled rapidly in water to create polymer nanoparticles(BASA-AA NPs)with a uniform size of 45 nm,no dye leakage,and great brightness(Φ=36%).Because of the enormous number of carboxyl groups on the surface of the particles,it can disperse extremely well in water,and can be used for the rapid detection of copper ion and cysteine(Cys)in pure water,with the limit of detection of 45 nmol/L.The fluorescent intensity of the nanoparticles will be greatly reduced after the introduction of copper ion,realizing the detection of copper ion with high selectivity and sensitivity.A composite probe made of BASA-AA NPs and copper ion can detect Cys in the range of 0.1-10μmol/L,with the detection limit of 84 nmol/L,due to the high binding capacity of the sulfhydryl group and copper ion.The method is simple and rapid in material synthesis and preparation,and shows high selectivity and sensitivity in pure water.

关 键 词：聚集诱导发光 两亲性聚合物 聚合物纳米粒子 铜离子检测 半胱氨酸检测 

分 类 号：O657.31[理学—分析化学] X832[理学—化学]