人体口腔中牙周病原菌测定  

Determination of periodontal pathogens in human oral cavity

作　　者：王鹏飞 陶春先[1] 张大伟[1] 李振庆[1] 山口佳则[2] WANG Pengfei;TAO Chunxian;ZHANG Dawei;LI Zhenqing;YAMAGUCHI Yoshinori(School of Opti-cal-Electrical and Computer Engineering,Key Laboratory of Medical Optical Instrument and Equipment of Minis-try of Education,University of Shanghai for Science and Technology,Shanghai 200093,China;Photonics Ad-vanced Research Center,Osaka University,Osaka 5650871,Japan)

机构地区：[1]上海理工大学光电信息与计算机工程学院,教育部医用光学仪器与设备重点实验室,上海200093 [2]大阪大学先进光子研究中心,大阪5650871,日本

出　　处：《分析试验室》2023年第4期465-469,共5页Chinese Journal of Analysis Laboratory

基　　金：上海市2018年度“科技创新行动计划”(18441900400)资助。

摘　　要：研究了多重聚合酶链式反应(PCR)和毛细管电泳技术(CE)快速测定口腔中牙龈卟啉单胞菌(PG)、福赛坦菌(TF)和齿垢密螺旋体(TD)3种牙周病致病菌的方法以及采样位置的影响。结果表明:采用CE分析DNA时,迁移时间与DNA长度呈线性关系,相关系数为0.9976;在0.5%羟乙基纤维素(HEC)分离介质中,可以在1.6 min内实现3种牙周病原菌PCR产物的高效分离。在牙周袋内不同位置处采样,其PCR产物也存在差异;CE在分析PCR产物时,电泳峰对应时间最高相对标准偏差为7.4%。通过分析荧光信号强度,可以推测出PG,TD和TF在牙龈液中的浓度分别为5.13×10^(-11),7.89×10^(-11)和4.87×10^(-11)ng/μL。The simultaneous detection of Porphyromonas gingivalis(PG),Tannerela forsythia(TF),and Treponema dentinarum(TD)based on multiplex polymerase chain reaction(PCR)and capillary electrophoresis(CE),and the effect of sampling position were investigated.Results demonstrated that there was a linear relationship between migration time and DNA length when DNA fragments were separated by capillary electrophoresis,and the correlation coefficient was 0.9976.In 0.5%hydroxyethyl cellulose(HEC),the PCR products of these periodontal pathogens could be separated within 1.6 min.When sampling at different positions in the periodontal pocket,the PCR products were also different.When analyzing PCR products by capillary electrophoresis,the maximum relative deviation of the peak time was 7.4%.Based on fluorescence intensity,it could be inferred that the concentrations of PG,TD and TF in the gingival fluid were 5.13×10^(-11),7.89×10^(-11)and 4.87×10^(-11)ng/μL,respectively.

关 键 词：毛细管电泳 聚合酶链式反应技术 牙周病原菌 

分 类 号：O657.8[理学—分析化学]