肠道湿热泄泻和寒湿泄泻“同病异治”的物质基础研究  

Material basis of the same disease and different treatment of intestinal damp heat diarrhea and cold damp diarrhea

作　　者：陈宝霞 任建明 周轲 李宏雅 牛淳 纪鹏[1] 华永丽[1] 魏彦明[1] CHEN Baoxia;REN Jianming;ZHOU Ke;LI Hongya;NIU Chun;JI Peng;HUA Yongli;WEI Yanming(College of Animal Medicine,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物医学院,兰州730070

出　　处：《中国实验动物学报》2023年第4期417-427,共11页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金项目(32260898);国家肉牛/牦牛产业技术体系(CARS-37);甘肃农业大学伏羲杰出人才培育计划(Gaufx-03J01)。

摘　　要：目的建立湿热、寒湿泄泻大鼠模型,采用白头翁汤与理中汤治疗湿热、寒湿泄泻模型大鼠,分析湿热、寒湿不同证型泄泻大鼠临床症状、细胞密度、氧化应激等指标的差异,为探索泄泻“同病异治”的治疗特色提供客观依据。方法将SD大鼠分为:空白组(CON组);湿热泄泻模型组(DHD组);湿热+白头翁汤组(DHB组);湿热+理中汤组(DHL组);寒湿泄泻模型组(CDD组);寒湿+理中汤组(CDL组);寒湿+白头翁汤组(CDB组)7个组。番泻叶+湿热、寒湿环境建立湿热、寒湿泄泻大鼠模型,采用白头翁汤和理中汤进行“以方测证”研究,记录各组大鼠的临床症状。HE染色观察脾组织病理学变化。AB-PAS染色观察结肠、回肠中杯状细胞,回肠中潘氏细胞密度变化。Western Blot检测大鼠结肠组织Nrf2、HO-1、AQP-4的表达量。结果(1)湿热、寒湿泄泻大鼠模型成功建立,湿热泄泻大鼠临床症状更明显。(2)白头翁汤、理中汤可有效改善湿热、寒湿泄泻大鼠的临床症状与脾病理组织变化。(3)泄泻大鼠肠杯状细胞、潘氏细胞密度均降低(P<0.01),湿热泄泻大鼠更明显。白头翁汤、理中汤可分别提高湿热、寒湿泄泻大鼠肠杯状细胞、潘氏细胞密度(P<0.01)。(4)泄泻大鼠结肠中Nrf2、HO-1、AQP-4的表达量降低(P<0.01),白头翁汤、理中汤能分别提高湿热、寒湿泄泻大鼠结肠组织中Nrf2、HO-1、AQP-4的表达量(P<0.01)。结论本研究成功建立了湿热、寒湿泄泻模型,其中湿热泄泻大鼠临床症状更明显。两种模型的脾病理组织变化以及肠黏膜杯状细胞和潘氏细胞密度均无显著性差异。相比湿热泄泻大鼠,寒湿泄泻大鼠抗氧化指标变化更明显。白头翁汤、理中汤分别用于治疗湿热、寒湿泄泻具有显著效果,而对于寒湿、湿热泄泻无疗效,为泄泻“同病异治”的研究提供了客观依据。Objective A rat model of damp heat and cold damp diarrhea was established and treated with Baitouweng and Lizhong decoctions to analyze differences in clinical symptoms,cell density,and oxidative stress indexes of diarrhea rats with different syndrome types of damp heat and cold damp diarrhea,and provide an objective basis to explore the treatment characteristics of diarrhea with the same disease and different treatment.Methods SD rats were divided into blank(CON),dampness heat diarrhea model(DHD),dampness heat+Baitouweng decoction(DHB),dampness heat+Lizhong decoction(DHL),cold dampness diarrhea model group(CDD),cold dampness+Lizhong decoction(CDL),and cold dampness+Baitouweng decoction group(CDB)groups.The rat model of damp heat and cold damp diarrhea was established by a senna leaf+damp heat and cold damp environment.Baitouweng and Lizhong decoctions were used in the prescription and syndrome test.Clinical symptoms of rats were recorded.HE was used to observe pathological changes in spleen tissues.The densities of goblet and Paneth cells in the colon and ileum were observed by AB-PAS staining.Nrf2,HO-1,and AQP-4 expression was detected by Western Blot.Results(1)The rat model of damp heat and cold damp diarrhea was successfully established,and the clinical symptoms of damp heat diarrhea rats was obvious.(2)Baitouweng and Lizhong decoctions effectively improved clinical symptoms and spleen pathological changes of damp heat and cold damp diarrhea rats.(3)The densities of goblet and Paneth cells were decreased in diarrhea rats(P<0.01),especially in damp heat diarrhea rats.Baitouweng and Lizhong decoction regulated the densities of goblet and Paneth cells in damp heat and cold damp diarrhea rats,respectively(P<0.01).(4)Nrf2,HO-1,and AQP-4 expression was decreased in the colon of diarrhea rats(P<0.01).Baitouweng and Lizhong decoctions increased Nrf2,HO-1,and AQP-4 expression in the colon of damp heat and cold damp diarrhea rats,respectively(P<0.01).Conclusions The model of damp heat and cold damp diarrhea was success

关 键 词：湿热泄泻 寒湿泄泻 白头翁汤 理中汤 

分 类 号：Q95-33[生物学—动物学]