豚草花粉-屋尘螨混合抗原诱导比格犬哮喘的探索性研究  

Exploratory study of asthma in beagle dogs induced by mixedantigens of ragweed pollen and house dust mite

作　　者：罗钰龙 陈迪非 陈焕杰 龙海斌 刘经纬 李时悦[2] 苏柱泉[2] LUO Yulong;CHEN Difei;CHEN Huanjie;LONG Haibin;LIU Jingwei;LI Shiyue;SU Zhuquan(Innovation Centre for Advanced Interdisciplinary Medicine,Key Laboratory of Biological Targeting Diagnosis,Therapy and Rehabilitation of Guangdong Higher Education Institutes,the Fifth Affiliated Hospital of Guangzhou Medical University,Guangzhou 510700;China.2.State Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,Guangzhou Institute of Respiratory Health,the First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510120;.3.National Canine Laboratory Animal Resource Centre,Guangzhou General Pharmaceutical Research Institute Co.,Ltd,Guangzhou 510240)

机构地区：[1]广州医科大学附属第五医院,前沿医学交叉研究中心,广东高校生物靶向诊治与康复重点实验室,广州510700 [2]广州医科大学附属第一医院,国家呼吸医学中心,广州呼吸健康研究院,呼吸疾病国家重点实验室,国家呼吸系统疾病临床医学中心,广州510120 [3]广州医药研究总院有限公司国家犬类实验动物资源库,广州510240

出　　处：《中国实验动物学报》2023年第4期437-444,共8页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(81770017);广东省教育厅高校特色创新团队(2021KCXTD028);广州市基础研究计划项目(202102020916);广州医科大学科研能力提升计划。

摘　　要：目的 本研究探索建立症状明显,稳定高效的比格犬哮喘模型。方法 将屋尘螨抗原与豚草花粉抗原配制成单一或混合抗原工作液。健康比格犬在出生后24 h内和第1、4、8周注射混合抗原致敏,第1~12周每天(每日雾化组)或每周2次(间断雾化组)雾化吸入豚草花粉抗原或混合抗原进行激发,第13周起每周1次雾化吸入豚草花粉抗原至第36周。在第36周和第48周进行气道反应性检测、肺泡灌洗液白细胞分类检测和病理分析考察哮喘造模效果。结果 第36周每日雾化组和间断雾化组比格犬气道均出现中性粒细胞炎症、嗜酸性粒细胞炎症和气道高反应性。第48周每日雾化组和间断雾化组的气道嗜酸性粒细胞炎症和每日雾化组的气道高反应性症状仍持续存在。两组比格犬均出现哮喘相关气道重塑现象。结论 使用豚草花粉抗原和屋尘螨抗原的混合抗原对新生犬进行致敏和激发,并加大吸入激发频次,可以建立比格犬哮喘模型。Objective To establish a stable and efficient asthma model in beagle dogs.Methods The antigen of ragweed short(Ambrosia elatia,RS)pollen or mixed antigens of both house dust mite(Dermatophagoides farinae,HDM)and RS pollen were prepared.Healthy beagle dogs were sensitized by injecting mixed antigens within 24 hours after birth and at 1,4,and 8 weeks.From week 1 to 12,RS pollen antigen or mixed antigens were inhaled every day(daily nebulization group,DN)or twice a week(intermittent nebulization group,IN)for airway challenge.From week 13,RS pollen antigen was inhaled once a week until week 36.At 36 and 48 weeks,an airway responsiveness test,differential counting of leukocytes in alveolar lavage fluid,and pathological analysis were carried out to evaluate the outcomes of asthma modeling.Results At 36 weeks,neutrophilic and eosinophilic inflammation in airways and airway hyperresponsiveness were observed in DN and IN groups.At 48 weeks,airway eosinophilic inflammation in DN and IN groups,and airway hyperresponsiveness in the DN group persisted.Asthma-related airway remodeling was observed in both groups.Conclusions An asthma model in beagle dogs can be established by sensitizing and challenging newborn dogs with mixed antigens of RS pollen and HDM,and increasing the frequency of antigen inhalation challenge.

关 键 词：比格犬 哮喘 豚草花粉 屋尘螨 动物模型 

分 类 号：Q95-33[生物学—动物学]