Pkd1^(f/f):Cre小鼠在自然状态下的发病情况研究  

Pathogenesis of Pkd1^(f/f):Cre mice in their natural state

作　　者：周卫民[1] 刘庆生[2] 吕东颖 陈方明[1] 朱科燕[1] 王德军[1] ZHOU Weimin;LIU Qingsheng;LYU Dongying;CHEN Fangming;ZHU Keyan;WANG Dejun(Zhejiang Chinese Medical University Laboratory Animal Research Center,Hangzhou 310053,China;Hangzhou Hosptial of Traditional Chinese Medicine,Hangzhou 310001)

机构地区：[1]浙江中医药大学动物实验研究中心/比较医学研究所,杭州310053 [2]杭州市中医院,杭州310001

出　　处：《中国实验动物学报》2023年第4期501-506,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：浙江省医药卫生科技计划(2018ky547)。

摘　　要：目的对Pkd1^(f/f):Cre转基因小鼠的发病特点和病理特征进行研究,为临床研究提供依据。方法选用自然状态下发病的Pkd1^(f/f):Cre转基因小鼠,通过对肝、肾组织进行解剖,观察超声多普勒下的肝形态,测定脏器系数,HE染色观察肝肾组织的病理情况,免疫组化分析肝肾PCNA、E-cadherin蛋白的表达变化。结果与Pkd1^(f/f)组相比,Pkd1^(f/f):Cre组的肝重量及系数极显著升高(P<0.01),体重在Pkd1^(f/f):Cre与Pkd1^(f/f)组间比较无差异;肾重量及系数在Pkd1^(f/f):Cre与Pkd1^(f/f)组间比较无显著性差异;其中与肝功能相关的因子ALT和AST的水平,Pkd1^(f/f):Cre组显著高于Pkd1^(f/f)组(P<0.01);而肾功能相关的因子BUN与CREA、UA水平在Pkd1^(f/f):Cre组与Pkd1^(f/f)组间无差异(P>0.05)。结论Pkd1^(f/f):Cre小鼠会发生肝囊肿,损害肝功能,但肾未出现囊肿的情况。Objective The pathogenesis and pathological characteristics of Pkd1^(f/f):Cre transgenic mice were studied,and the transgenic mice were used for clinical research.Methods Diseased Pkd1^(f/f):Cre transgenic mice in their natural state were selected,liver and kidney tissues were collected,liver morphology under Doppler ultrasound was observed,the organ coefficient was determined,and the pathological conditions of liver and kidney tissues were observed by HE staining.Expression changes of PCNA and E-cadherin proteins in the liver and kidney were analyzed by histochemistry.Results Compared with the Pkd1^(f/f) group,the liver weight and coefficient of the Pkd1^(f/f):Cre group were significantly increased(P<0.01).There was no difference in body weight between Pkd1^(f/f):Cre and Pkd1^(f/f) groups.There was no difference in the kidney weight and coefficient between Pkd1^(f/f):Cre and Pkd1^(f/f) groups.ALT and AST levels related to liver functions were significantly higher in the Pkd1^(f/f):Cre group than in the Pkd1^(f/f):Cre group(P<0.01).There was no difference in the levels of function-related factors BUN,CREA,and UA between Pkd1^(f/f):Cre and Pkd1^(f/f) groups(P>0.05).Conclusions Pkd1^(f/f):Cre mice develop liver cysts and disrupted liver functions,but no cysts appear in the kidneys.

关 键 词：polycystin1基因 肝 肾 肝囊肿 

分 类 号：Q95-33[生物学—动物学]