西藏小型猪颈动脉粥样硬化模型的建立及评价研究  

Establishment and disease characteristics of a carotid atherosclerosis model in Tibet mini-pigs

作　　者：徐雁云 潘永明[2] 黄俊杰 屠海烨 郁晨 陈民利[2] 徐孝平[2] XU Yanyun;PAN Yongming;HUANG Junjie;TU Haiye;YU Chen;CHEN Minli;XU Xiaoping(School of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China;Laboratory Animal Research Center/Institute of Comparative Medicine,Zhejiang Chinese Medical University,Hangzhou 310053)

机构地区：[1]浙江中医药大学药学院,杭州310053 [2]浙江中医药大学动物实验研究中心/比较医学研究所,杭州310053

出　　处：《中国实验动物学报》2023年第3期287-295,共9页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金面上项目(31970514);浙江省公益技术研究计划(LGD20C040004)。

摘　　要：目的采用高脂饮食联合左侧颈动脉球囊损伤术建立西藏小型猪颈动脉粥样硬化(carotid atherosclerosis,CAS)模型,观察西藏小型猪CAS模型的血液生化、影像学及病理学特点。方法10只雄性西藏小型猪随机分为两组,正常组饲喂正常饲料,模型组持续饲喂高脂饲料且2周后进行左侧颈动脉球囊损伤术,造模周期为12周。分别在高脂饲喂前(-2周)、球囊损伤(0周)及术后2周、6周、10周检测其体重和血液生化等指标,并在术后10周进行超声、血流多普勒、血管造影等影像学观测。动物安乐死后取左侧颈动脉进行HE、Masson和油红“O”染色,观察其形态、炎症、纤维化和脂质沉积等情况,分析西藏小型猪的病变特点。结果西藏小型猪CAS模型的多项血液生化指标与临床相似;影像学结果显示CAS模型左颈动脉血管显著狭窄且阻力增大,导致血流速度减小;病理观察有明显炎症细胞浸润、胶原纤维增生、血管壁增厚和脂质沉积等,与临床表现近似。结论西藏小型猪CAS模型与临床特点相似,模型易感且稳定,是适用于研究CAS的良好的理想模型。Objective By combining a high fat diet with balloon injury to the left carotid artery,a carotid atherosclerosis(CAS)model in Tibetan miniature pigs was established,and hematobiochemical,imaging,and pathological features of the CAS model were analyzed.Methods Ten male Tibetan miniature pigs were randomly divided into two groups,one of which received a normal diet,whereas the other had an balloon injury to the left carotid artery after 2 weeks while being fed a high fat diet.The modeling period was 12 weeks.Body weight and blood biochemical indexes were measured before high fat feeding(-2 weeks)and balloon injury(0 weeks)and after 2,6,and 10 weeks.Ultrasound,Doppler flow imaging,and angiography were performed at 10 weeks after the operation.To investigate morphology,inflammation,fibrosis,and lipid deposition,and lesion characteristics of Tibetan mini-pigs,the left carotid artery of the animals was stained with HE,Masson,and oil red O after euthanasia.Results Numerous blood biochemical parameters in the CAS model were similar to the clinical parameters.Imaging findings revealed significant narrowing and increased resistance of the left carotid artery in the CAS model.Resultsing in reduced blood flow velocity.Pathological observations revealed significant inflammatory cell infiltration,collagen fiber proliferation,vessel wall thickening,and lipid deposition,which were similar to clinical manifestations.Conclusions The CAS model in Tibet miniature pig is a an ideal model to investigate CAS because it is sensitive,stable,and resembles clinical aspects.

关 键 词：西藏小型猪 颈动脉粥样硬化 动物模型 

分 类 号：Q95-33[生物学—动物学]