梅花鹿鞭与马鹿鞭PCR-RFLP鉴别研究  

Identification of Sika deer penis and Red deer penis by PCR-RFLP

作　　者：周童 吴楠 孟媛 姜菲菲 李佩瑶 张英 裴笑仙 艾金霞 ZHOU Tong;WU Nan;MENG Yuan;JIANG Feifei;LI Peiyao;ZHANG Ying;PEI Xiaoxian;AI Jinxia(School of Medical Technology,Beihua University,Jilin City,Jilin Province,132013,China)

机构地区：[1]北华大学医学技术学院,吉林吉林132013

出　　处：《吉林医药学院学报》2023年第2期81-86,共6页Journal of Jilin Medical University

基　　金：吉林省科技发展计划项目(20200404152YY,20210204185YY);北华大学医学技术学院“晨裕”科研创新项目(BH007)。

摘　　要：目的建立一种应用聚合酶链式反应-限制性片段长度多态性技术(PCR-RFLP)鉴别梅花鹿鞭与马鹿鞭的方法。方法根据梅花鹿与马鹿的细胞色素b基因序列的差异,设计并选取DNA限制性内切酶MseI来区分梅花鹿鞭和马鹿鞭。采用改良SDS法提取样本基因组DNA,经PCR扩增后进行RFLP分析,并优化PCR-RFLP反应体系及反应条件,用优化的PCR-RFLP体系对市售鹿鞭样本进行鉴定。结果经PCR-RFLP分析,MseI可切割梅花鹿与马鹿Cyt b片段,前者被切成2个片段,后者被切成3个片段,从而将两者进行鉴别。结论本实验建立的PCR-RFLP方法可准确鉴别梅花鹿鞭与马鹿鞭。Objective To establish a method for identifying sika deer penis and red deer penis by polymerase chain reaction-restriction fragment length polymorphism.Methods The DNA restriction endonuclease MseI was designed and selected to distinguish the sika deer penis from the red deer penis based on the difference in cytochrome b gene sequences between sika deer and red deer.The genomic DNA of samples was extracted by improved SDS method,amplified by PCR and analyzed by RFLP,and the PCR-RFLP reaction system and reaction conditions were optimized.The deer penis samples were identified by the optimized PCR-RFLP system.Results By PCR-RFLP analysis,MseI can cut cytochrome b fragments of sika deer and red deer,the former is cut into two fragments and the latter into three fragments,so as to identify them.Conclusion The PCR-RFLP method established in this experiment can accurately identify the sika deer penis from the red deer penis.

关 键 词：鹿鞭 聚合酶链式反应-限制性片段长度多态性技术 梅花鹿鞭 马鹿鞭 

分 类 号：R282.5[医药卫生—中药学]