丹蛭降糖胶囊对糖尿病牙周炎大鼠牙周组织的干预研究  

Intervention of Danzhi Jiangtang Capsule on the Regulation of Periodontal Tissue in Diabetic Periodontitis Rats

作　　者：董蕾[1] 方朝晖[2] 胡宜成 陶玮[1] DONG Lei;FANG Zhaohui;HU Yicheng;TAO Wei(Department of Stomatology,Anhui Medical College,Hefei 230601,China;Department of Endocrinology,First Affiliated Hospital of Anhui University of Traditional Chinese Medicine,Hefei 230000,China)

机构地区：[1]安徽医学高等专科学校口腔医学院,安徽合肥230601 [2]安徽中医药大学第一附属医院内分泌科,安徽合肥230000

出　　处：《皖西学院学报》2023年第2期62-68,共7页Journal of West Anhui University

基　　金：安徽省高校自然科学研究重点项目(KJ2015A429);安徽省高校学科(专业)拔尖人才学术资助项目(gxbjZD2021102)。

摘　　要：为研究丹蛭降糖胶囊(DJC)对糖尿病牙周炎的治疗效果及潜在机制,以100只大鼠建立糖尿病牙周炎模型,并随机分为5组,测量所有大鼠的空腹血糖(FPG)、C肽和糖化血红蛋白(HbA1c)。通过定量聚合酶链反应(Q-PCR)和蛋白质印迹(WB)评估炎性细胞因子的mRNA和蛋白质表达水平。通过WB测量Wnt/β-连环蛋白通路的蛋白质表达水平。结果发现:经过DJC处理,大鼠的血糖和C肽浓度逐渐降低,CEJ-A的差距和PDL的百分比逐渐减小,HbA1c逐渐升高,牙周组织中单核细胞和白细胞数量明显减少(P<0.05)。应用DJC后Wnt1和β-连环蛋白的蛋白水平显著升高,而SOST和DDK1的蛋白水平明显降低。BGP的表达水平上升,而TNF-α、IFN-γ和MMP-3明显降低。Wnt/β-连环蛋白抑制剂可以将所有结果逆转。结论:DJC能通过Wnt/β-连环蛋白信号通路减轻牙周组织炎症,从而改善糖尿病牙周炎大鼠的高血糖状态和远端牙槽骨及牙槽骨分叉区的骨丢失。To elucidate whether Danzhi Jiangtang capsule(DJC)has treatment effects on diabetic periodontitis and the potential mechanism.100 rats were used to establish the model of diabetic periodontitis,and were randomly divided into 5 groups,fasting plasma glucose(FPG),C-peptide and glycosylated hemoglobin(HbA1c)of rats were measured.The mRNA and protein levels of inflammatory cytokines were evaluated by quantitative polymerase chain reaction(Q-PCR)and Western blotting(WB).Protein levels of Wnt/β-catenin signaling molecules were measured by WB.After DJC treatment,the blood glucose and C-peptide concentrations were gradually reduced,with gradually decreased distance of CEJ-A and the percentage of PDL,as well as gradually increased HbA1c.The number of monocytes and leukocytes in periodontal tissue was markedly decreased(P<0.05).Protein levels of Wnt1 andβ-catenin were obviously up-regulated with DJC treatment,while the SOST and DDK1 were markedly own-regulated with DJC treatment.The expression levels of BGP increased,while TNF-α,IFN-γ,and MMP-3 decreased significantly.All these changes could be reversed by Wnt/β-catenin inhibitor.CONCLUSION:DJC can improve the hyperglycemia and both distal alveolar bone loss and alveolar bone loss in furcation area of diabetic periodontitis rats by reducing the inflammation of gingival tissue through Wnt/β-catenin signaling.

关 键 词：高血糖 牙周炎 牙槽骨丢失 WNT信号通路 Β-连环蛋白 丹蛭降糖胶囊 

分 类 号：R977.15[医药卫生—药品]