鸭胆碱激酶α(CHKA)基因结构及功能的生物信息学分析  

Bioinformatic Analysis of the Structure and Function of Duck Choline Kinase α(CHKA) Gene

作　　者：邱桂如 薛百玲 税斐 曹程程 周旋 潘子婷 冯璐 金四华[2] QIU Guiru;XUE Bailing;SHUI Fei;CAO Chengcheng;ZHOU Xuan;PAN Ziting;FENG Lu;JIN Sihua(School of Public Health,Anhui Medical University,Hefei 230032,China;College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China)

机构地区：[1]安徽医科大学公共卫生学院,安徽合肥230032 [2]安徽农业大学动物科技学院,安徽合肥230036

出　　处：《皖西学院学报》2023年第2期69-73,144,共6页Journal of West Anhui University

基　　金：高校优秀青年人才支持计划重点项目(gxyqZD2022017);安徽省家禽产业体系专项经费(AHCYJSTX-06);安徽省重点研究计划项目(202004a06020049)。

摘　　要：基于生物基因组学数据库,对鸭胆碱激酶α(Choline kinaseα,CHKA)基因进行生物信息学分析,同时研究CHKA的定位。结果显示,鸭CHKA共编码氨基酸477个,蛋白分子式为C_(2304)H_(3565)N_(605)O_(657)S_(22),相对分子质量为50957.63,理论等电点为6.70,脂肪系数为77.07。同源性分析发现,其与鹅的同源性为99.3%,与疣鼻天鹅的同源性为99.1%,与红色原鸡的同源性为93.7%,与其他动物的同源性为74%~92%。CHKA蛋白无信号肽,具有2个低复杂度结构域,二级结构以α螺旋为主。亚细胞定位表明,CHKA在胞外含量最高,在细胞核和胞浆中含量较多,在细胞其他位置也有分布。预测结果为进一步研究鸭CHKA基因及其编码蛋白的结构和生物学功能提供参考依据。The Choline kinaseα(CHKA)gene in duck was analyzed by bioinformatics based on Biogenomics database,and the location of CHKA was also studied.The results showed that duck CHKA encoded 477 amino acids,protein molecular formula C_(2304)H_(3565)N_(605)O_(657)S_(22),relative molecular weight 50957.63,theoretical isoelectric point 6.70,fat coefficient 77.07.Homology analysis found that it has 99.3%homology with geese,99.1%homology with mute swans,93.7%homology with red prairie fowl,and 74%to 92%homology with other animals.CHKA protein has no signal peptide,two low complexity domains,and its secondary structure is dominated byαhelix.Subcellular localization showed that the extracellular content of CHKA was the highest in the nucleus and cytoplasm,and also distributed in other locations of the cell.The prediction results will provide important references for further study of the structure and biological function of duck CHKA gene and its encoded protein.

关 键 词：鸭 CHKA基因 生物信息学分析 同源性 

分 类 号：Q814.1[生物学—生物工程]