Fast integration and accumulation of beneficial breeding alleles through an AB–NAMIC strategy in wheat  

作　　者：Chengzhi Jiao Chenyang Hao Tian Li Abhishek Bohra Lanfen Wang Jian Hou Hongxia Liu Hong Liu Jing Zhao Yamei Wang Yunchuan Liu Zhiwei Wang Xin Jing Xiue Wang Rajeev K.Varshney Junjie Fu Xueyong Zhang 

机构地区：[1]The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [2]State Key Laboratory of Crop Genetics and Germplasm Enhancement,Cytogenetics Institute,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China [3]Centre for Crop&Food Innovation,WA State Agricultural Biotechnology Centre,Food Futures Institute,Murdoch University,Perth,WA 6150,Australia [4]Smartgenomics Technology Institute,Tianjin 301700,China

出　　处：《Plant Communications》2023年第3期66-79,共14页植物通讯（英文）

基　　金：supported by the National Key Research and Development Program of China(2022YFD1201503 and 2016YFD0100302);the National Major Agricultural Science and Technology Project(NK2022060101).

摘　　要：Wheat(Triticum aestivum)is among the most important staple crops for safeguarding the food security of the growing world population.To bridge the gap between genebank diversity and breeding programs,we developed an advanced backcross-nested association mapping plus inter-crossed population(AB-NAMIC)by crossing three popular wheat cultivars as recurrent founders to 20 germplasm lines from a mini core collection.Selective backcrossing combined with selection against undesirable traits and extensive crossing within and between sub-populations created new opportunities to detect unknown genes and increase the frequency of beneficial alleles in the AB-NAMIC population.We performed phenotyping of 590 AB-NAMIC lines and a natural panel of 476 cultivars for six consecutive growing seasons and genotyped these 1066 lines with a 660K SNP array.Genome-wide association studies of both panels for plant development and yield traits demonstrated improved power to detect rare alleles and loci with medium genetic effects in AB-NAMIC.Notably,genome-wide association studies in AB-NAMIC detected the candidate gene TaSWEET6-7B(TraesCS7B03G1216700),which has high homology to the rice SWEET6b gene and exerts strong effects on adaptation and yield traits.The commercial release of two derived AB-NAMIC lines attests to its direct applicability in wheat improvement.Valuable information on genome-wide association studymapping,candidate genes,and their haplotypes for breeding traits are available through WheatGAB.Our research provides an excellent framework for fast-tracking exploration and accumulation of beneficial alleles stored in genebanks.

关 键 词：WHEAT GENEBANK AB-NAMIC genome-wide association studies GWAS beneficial alleles genomics-assisted breeding WheatGAB 

分 类 号：S512.1[农业科学—作物学]