A novel ambigrammatic mycovirus,PsV5,works hand in glove with wheat stripe rust fungus to facilitate infection  

作　　者：Yanhui Zhang Xiaofei Liang Mengxin Zhao Tuo Qi Hualong Guo Jing Zhao Jie Zhao Gangming Zhan Zhensheng Kang Li Zheng 

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection,Northwest A&F University,Yangling,Shaanxi 712100,China [2]Sanya Nanfan Research Institute of Hainan University,Hainan Yazhou Bay Seed Laboratory,Sanya 572025,China [3]Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests,Ministry of Education and School of Plant Protection,Hainan University,Haikou,Hainan 570228,China [4]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,State Key Laboratory of Hybrid Rice,Key Laboratory of Major Crop Diseases&Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin,Rice Research Institute,Sichuan Agricultural University at Wenjiang,Chengdu,Sichuan 611130,China

出　　处：《Plant Communications》2023年第3期112-125,共14页植物通讯（英文）

基　　金：supported by the National Natural Science Foundation of China(32260648);the Hainan Province Key R&D Project(ZDYF2022XDNY242);the Scientific Research Foundation for Advanced Talents,Hainan University(KYQD(ZR)1873);the National Key Research and Development Program of China(2021YFD1401000);the 111 Project from the Ministry of Education of China(B07049).

摘　　要：Here we describe a novel narnavirus,Puccinia striiformis virus 5(PsV5),from the devastating wheat stripe rust fungus P.striiformis f.sp.tritici(Pst).The genome of PsV5 contains two predicted open reading frames(ORFs)that largely overlap on reverse strands:an RNA-dependent RNA polymerase(RdRp)and a reverseframe ORF(rORF)with unknown function.Protein translations of both ORFs were demonstrated by immune technology.Transgenic wheat lines overexpressing PsV5(RdRp-rORF),RdRp ORF,or rORF were more susceptible to Pst infection,whereas PsV5-RNA interference(RNAi)lines were more resistant.Overexpression of PsV5(RdRp-rORF),RdRp ORF,or rORF in Fusarium graminearum also boosted fungal virulence.We thus report a novel ambigrammatic mycovirus that promotes the virulence of its fungal host.The results are a significant addition to our understanding of virosphere diversity and offer insights for sustainable wheat rust disease control.

关 键 词：Puccinia striiformis f.sp.tritici MYCOVIRUS ambigrammatic virulence-enhancing 

分 类 号：S435[农业科学—农业昆虫与害虫防治]