A chromosome-scale genome assembly of Artemisia argyi reveals unbiased subgenome evolution and key contributions of gene duplication to volatile terpenoid diversity  

作　　者：Hongyu Chen Miaoxian Guo Shuting Dong Xinling Wu Guobin Zhang Liu He Yuannian Jiao Shilin Chen Li Li Hongmei Luo 

机构地区：[1]Key Laboratory of Chinese Medicine Resources Conservation,State Administration of Traditional Chinese Medicine of the People’s Republic of China,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China [2]Shandong University of Traditional Chinese Medicine,Jinan 250355,China [3]State Key Laboratory of Crop Biology,Shandong Agricultural University,Taian 271018,China [4]College of Agronomy,Shandong Agricultural University,Taian 271018,China [5]State Key Laboratory of Systematic and Evolutionary Botany,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China [6]University of the Chinese Academy of Sciences,Beijing 100049,China [7]Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China [8]Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China [9]Robert W.Holley Center for Agriculture and Health,USDA-ARS,Cornell University,Ithaca,NY 14853,USA [10]Plant Breeding and Genetics Section,School of Integrative Plant Science,Cornell University,Ithaca,NY 14853,USA

出　　处：《Plant Communications》2023年第3期228-243,共16页植物通讯（英文）

基　　金：supported by the National Natural Science Foundation of China(81973422 and 31570302);the Chinese Academy of Medical Sciences(CAMS)Innovation Fund for Medical Sciences(2021-I2M-1-071).

摘　　要：Artemisia argyi Le´vl.et Vant.,a perennial Artemisia herb with an intense fragrance,is widely used in traditional medicine in China and many other Asian countries.Here,we present a chromosome-scale genome assembly of A.argyi comprising 3.89 Gb assembled into 17 pseudochromosomes.Phylogenetic and comparative genomic analyses revealed that A.argyi underwent a recent lineage-specificwhole-genomeduplication(WGD)event after divergence fromArtemisia annua,resulting in two subgenomes.Wedeciphered the diploid ancestral genome of A.argyi,and unbiased subgenome evolution was observed.The recent WGD led to a large number of duplicated genes in the A.argyi genome.Expansion of the terpene synthase(TPS)gene family through various types of gene duplication may have greatly contributed to the diversity of volatile terpenoids in A.argyi.In particular,we identified a typical germacrene D synthase gene cluster within the expanded TPS gene family.The entire biosynthetic pathways of germacrenes,(+)-borneol,and(+)-camphor were elucidated in A.argyi.In addition,partial deletion of the amorpha-4,11-diene synthase(ADS)gene and loss of function of ADS homologs may have resulted in the lack of artemisinin production in A.argyi.Our study provides newinsights into the genome evolution of Artemisia and lays a foundation for further improvement of the quality of this important medicinal plant.

关 键 词：Artemisia argyi subgenome evolution gene duplication terpene synthase germacrene synthase nonartemisinin production 

分 类 号：Q94[生物学—植物学]