Sustainable biosynthesis of valuable diterpenes in microbes  

作　　者：Yanbin Liu Xixian Chen Congqiang Zhang 

机构地区：[1]Singapore Institute of Food and Biotechnology Innovation(SIFBI),Agency for Science Technology and Research(A∗STAR),31 Biopolis Way,Level 6 Nanos building,Singapore 138669,Singapore

出　　处：《Engineering Microbiology》2023年第1期65-79,共15页工程微生物学（英文）

基　　金：supported by the Agency for Science,Technology and Research(A∗STAR),Singapore:AME Young Individual Research Grant(YIRG)A2084c0064(Dr.Congqiang Zhang)and A1984c0040(Dr.Xixian Chen);A∗STAR Central Strategic Fund for the Singa-pore Integrative Biosystems and Engineering Research(SIBER)Program C211917009.

摘　　要：Diterpenes,or diterpenoids,are the most abundant and diverse subgroup of terpenoids,the largest family of sec-ondary metabolites.Most diterpenes possess broad biological activities including anti-inflammatory,antiviral,anti-tumoral,antimicrobial,anticancer,antifungal,antidiabetic,cardiovascular protective,and phytohormone activities.As such,diterpenes have wide applications in medicine(e.g.,the anticancer drug Taxol and the antibiotic pleuromutilin),agriculture(especially as phytohormones such as gibberellins),personal care(e.g.,the fragrance sclareol)and food(e.g.,steviol glucosides as low-calorie sweeteners)industries.Diterpenes are biosyn-thesized in a common route with various diterpene synthases and decoration enzymes like cytochrome P450 oxidases,glycosidases,and acyltransferases.Recent advances in DNA sequencing and synthesis,omics analysis,synthetic biology,and metabolic engineering have enabled efficient production of diterpenes in several chassis hosts like Escherichia coli,Saccharomyces cerevisiae,Yarrowia lipolytica,Rhodosporidium toruloides,and Fusarium fujikuroi.This review summarizes the recently discovered diterpenes,their related enzymes and biosynthetic pathways,particularly highlighting the microbial synthesis of high-value diterpenes directly from inexpensive carbon sources(e.g.,sugars).The high titers(>4 g/L)achieved mean that some of these endeavors are reaching or close to commercialization.As such,we envisage a bright future in translating microbial synthesis of diterpenes into commercialization.

关 键 词：Diterpene/diterpenoid Secondary metabolites Synthetic biology Metabolic engineering BIOSYNTHESIS 

分 类 号：O62[理学—有机化学]