Carposome productivity of Pleurotus ostreatus and Pleurotus eryngii growing on agro-industrial residues enriched with nitrogen,calcium salts and oils  

作　　者：Marianna Dedousi Eirini-Maria Melanouri Panagiota Diamantopoulou 

机构地区：[1]Laboratory of Edible Fungi,Institute of Technology of Agricultural Products(ITAP),Hellenic Agricultural Organization-Dimitra,1 S.Venizelou Str,14123 Lykovryssi,Greece [2]Laboratory of Food Microbiology and Biotechnology,Department of Food Science and Nutrition,Agricultural University of Athens,75 Iera Odos Str,11855 Athens,Greece

出　　处：《Carbon Resources Conversion》2023年第2期150-165,共16页碳资源转化（英文）

基　　金：the European Regional Development Fund of the European Union and Greek national funds(European Social Fund-ESF)through the Operational Program Competitiveness,Entrepreneurship and Innovation,under the call RESEARCH-CREATE-INNOVATE(project code:Τ1EDK-05027),scientifically coordinated by the Hellenic Agricultural Organization-Dimitra(Institute of Technology of Agricultural Products/Laboratory of Edible Fungi).

摘　　要：The suitability of the abundant agro-industrial residues wheat straw(WS;control),barley and oats straw(BOS)and rice husk(RH),supplemented with various sources of oils(sunflower,corn oil),nitrogen(peptone,yeast extract)and calcium salts(CaSO_(4)⋅2H_(2)O,CaCl2),as novel substrates in solid-state fermentation of selected Pleurotus ostreatus and P.eryngii mushrooms was investigated.The possible effect of different additives on mycelial growth rate,biomass production and endoglucanase,laccase and lipase biosynthesis were evaluated.Moreover,their impact on essential cultivation aspects(earliness,total mushroom yield,biological efficiency)and carposome quality parameters(weight,morphological characteristics)was assessed.Both fungi showed their highest growth rates on BOS substrates and the most positive implementation was CaSO_(4)⋅2H_(2)O 6%w/w(Kr=9.58 mm/day;P.ostreatus,Kr=9.42 mm/day;P.eryngii),while different additives led to enhancement of biomass production.Pleurotus species demonstrated minimal levels of endoglucanase activity,with values ranging from 0.01 to 0.42 U/g of dry weight,regardless of the substrate and the stage of colonization.On the contrary,the maximum values of laccase activity were observed at 50%of colonization on BOS and RH,while supplementation with nitrogen and calcium sources positively affected its biosynthesis.P.ostreatus and P.eryngii cultivated on BOS supplemented with peptone at 2 and 5%w/w,synthesized significant laccase amounts,i.e.,12,165.78 and 8,624.55 U/g d.w.,respectively.Satisfactory amounts of lipase were produced,especially in substrates supplemented with sunflower 2%w/w,in quantities up to 1.42 U/g d.w.,whereas the highest lipase activity was achieved by P.eryngii on WS supplemented with corn oil at 2%w/w,with a value of 4.25 U/g d.w.being recorded.Regarding fermentation of Pleurotus species in polypropylene bags,WS and BOS supported faster colonization and shorter earliness period than RH substrates,whereas supplementation did not seem to affect these culture parameters.Fu

关 键 词：Oyster mushrooms RESIDUES Additives Biomass ENDOGLUCANASE LACCASE LIPASE Weight 

分 类 号：O62[理学—有机化学]