牛奶冻干粉中阿莫西林基体标准物质研制  

Development of a matrix reference material for amoxicillin in lyophilized bovine milk

作　　者：刘天和 魏宁[1] 武彩红[1] 张郁 刘秋慧 LIU Tian-he;WEI Ning;WU Cai-hong;ZHANG Yu;LIU Qiu-hui(College of Veterinary Medicine,Jiangsu Agri-animal Husbandry Vocational College,Taizhou,Jiangsu 225300,China)

机构地区：[1]江苏农牧科技职业学院动物医学院,江苏泰州225300

出　　处：《食品与机械》2023年第4期55-60,共6页Food and Machinery

基　　金：江苏农牧科技职业学院校级科研项目(编号:NSF2021ZR06);江苏省高校大学生创新创业训练计划项目(编号:202112806003Y)。

摘　　要：目的:为了给牛奶中阿莫西林残留分析提供量值溯源的依据,同时也为保障国家和各地区牛奶中阿莫西林监测工作的有效实施及打击阿莫西林的滥用提供量值标准。方法:选取两头健康中产奶牛肌肉注射阿莫西林,药物自然代谢一段时间后采集含有目标药物阿莫西林的牛奶,通过真空冷冻干燥技术将其制成含有阿莫西林的牛奶基体标准物质。对制备好的牛奶冻干粉基体标准物质进行均匀性检验、稳定性检验、定值、不确定度评估。结果:在均匀性检验中,获得的F值<F 0.05,表明制备的牛奶冻干粉阿莫西林基体标准物质均匀性良好;在稳定性检验中,短期稳定性检验(25,4,-20℃;2周)和长期稳定性检验(4,-20℃;6个月)结果显示其稳定性良好;通过8家实验室对标准物质进行联合定值,最终定值结果为4.05μg/kg,总不确定度为0.2μg/kg。结论:研制的牛奶冻干粉中阿莫西林基体标准物质的均匀性、稳定性良好,可成为牛奶中阿莫西林量值溯源的载体,并能广泛应用于牛奶中阿莫西林检测的方法学验证与质量控制。Objective:This study aimed to provide the basis of quantity traceability for the analysis of amoxicillin residues in milk.At the same time,it can also provide quantity standards for ensuring the effective implementation of amoxicillin monitoring in milk and combating the abuse of amoxicillin in the country and regions.Methods:Two healthy middle-class dairy cows were injected with amoxicillin intramuscularly.After the natural metabolism of the drug for a period of time,the milk containing the target drug amoxicillin was collected and made into milk matrix reference material containing amoxicillin by vacuum freeze-drying technology.The uniformity test,stability test,fixed value and uncertainty evaluation of the prepared milk lyophilized powder matrix reference material were carried out.Results:In the uniformity test,the F value obtained is<F 0.05,The results showed that the preparation of the standard milk powder was homogeneous;In the stability test,the short-term stability test(25,4,-20℃;2 weeks)and long-term stability test(4,-20℃;6 months)showed good stability;The reference materials were jointly calibrated by 8 laboratories,and the final calibration result was 4.05μg/kg,the total uncertainty being 0.2μg/kg.Conclusion:The prepared amoxicillin matrix reference material in milk freeze-dried powder has good uniformity and stability,which can be used as a carrier to trace the amount of amoxicillin in milk,and can be widely used in the methodology verification and quality control of amoxicillin detection in milk.

关 键 词：基体标准物质 冷冻干燥 牛奶 阿莫西林 检测 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]