牡蛎酶解产物对产后缺乳症泌乳功能改善作用探究  

Study on the effect of Oyster hydrolysates on improving Lactation function in Postpartum hypogalactism

作　　者：韦雪 秦小明 谌素华 张锴佳 林海生 郑惠娜 高加龙 WEI Xue;QIN Xiaoming;CHEN Suhua;ZHANG Kaijia;LIN Haisheng;ZHENG Huina;GAO Jialong(College of Food Science and Technology,Guangdong Ocean University,National Research and Development Branch Center for Shellfish Processing(Zhanjiang),Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety,Guangdong Province Engineering Laboratory for Marine Biological Products,Guangdong Provincial Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing,Zhanjiang 524088,China;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian 116034,China)

机构地区：[1]广东海洋大学食品科技学院,国家贝类加工技术研发分中心(湛江),广东省水产品加工与安全重点实验室,广东省海洋生物制品工程实验室,广东省亚热带果蔬加工科技创新中心,广东湛江524088 [2]海洋食品精深加工关键技术省部共建协同创新中心(大连工业大学),辽宁大连116034

出　　处：《热带海洋学报》2023年第2期141-152,共12页Journal of Tropical Oceanography

基　　金：财政部和农业农村部国家现代农业产业技术项目(CARS-49)。

摘　　要：文章以香港牡蛎(Crassostrea hongkongensis)为原料制备牡蛎酶解产物(oyster hydrolysis, OH),分析其基本营养成分和氨基酸组成,并探究其对甲磺酸溴隐亭致产后缺乳症大鼠的泌乳功能改善作用。结果表明:OH粗蛋白含量较高,其次是总糖,而脂肪含量较低,肽含量为33.4%;OH中必需氨基酸占总氨基酸的39.8%,且含有较高比例的支链氨基酸。动物试验结果表明:相较于模型组, OH高剂量组大鼠泌乳量显著增加(p <0.05)且优于阳性对照组;OH各剂量组均极显著提高了缺乳大鼠乳腺组织器官指数(p <0.01),其效果接近于阳性对照组,但未能恢复到正常水平;苏木精–伊红染色观察结果显示, OH各剂量组乳腺组织腺泡较多,腺泡呈现圆形且充盈,内有较多的分泌物,腺泡有明显扩大增生,相较于模型组,有明显的恢复;与模型组比较, OH各剂量组血清和乳腺组织催乳素(prolactin, PRL)、催乳素受体(prolactin receptor, PRLR)含量显著提高(p <0.05),说明OH能够促进缺乳大鼠催乳素分泌,而血清多巴胺(dopamine, DA)的含量极显著降低(p <0.01),表明OH能够降低由溴隐亭导致的多巴胺含量过高。此外, OH能显著提高乳腺组织中乳糖(lactose, LC)、甘油三酯(triglyceride, TG)含量(p <0.05),提高了乳汁营养成分;OH各剂量组血液中平均血红蛋白(mean corpuscular hemoglobin concentration, MCHC)、红细胞(red blood cell,RBC)的数量也显著增加(p<0.05),对产后气血不足有一定的改善作用。因此,OH能够改善缺乳症状,提高产后泌乳量,具有促进泌乳的潜力。In this paper,oyster hydrolysis products were prepared from Crassostrea hongkongensis as raw materials,and the basic nutrition and amino acid composition of oyster hydrolysis products were analyzed,and the improvement effect of oyster hydrolysis products on the lactation function of rats with postpartum lactation caused by Bromocriptine mesylate was explored.The results showed that protein content of oyster hydrolysis products was higher,which is followed by sugar,fat content was lower,and peptide content was 33.4%.The essential amino acids in oyster hydrolysis products accounted for 39.8%of the total amino acids and contained a high proportion of branched amino acids.The results of animal experiment showed that compared with model group,the lactation volume of rats in oyster hydrolysis products of high-dose group was significantly increased(p<0.05),which was better than that in the positive control group.The index of mammary tissue and organ in each oyster hydrolysis products dose group was significantly increased(p<0.01),which was close to the positive control group,but did not return to normal level.HE staining showed that there were more acinus in mammary gland tissues in all oyster hydrolysis products dose groups,which were round and full with more secretions,and the acinus had obvious enlargement and hyperplasia.Compared with the model group,the acinus had obvious recovery.Compared with model group,the contents of prolactin(PRL)and prolactin receptor(PRLR)in serum and mammary tissue of oyster hydrolysis products groups were largely increased(p<0.05),indicating that oyster hydrolysis products could promote the secretion of prolactin in lactation-deficient rats,while the content of serum dopamine(DA)was greatly decreased(p<0.01).These results showed that oyster hydrolysis products can reduce the high level of dopamine induced by bromocystine.In addition,oyster hydrolysis products strongly increased the contents of lactose(LC)and triglyceride(TG)in breast tissue(p<0.05),and improved the nutritional composition

关 键 词：牡蛎酶解产物 产后缺乳 甲磺酸溴隐亭 泌乳量 

分 类 号：P745.3[天文地球—海洋生物学]