堆肥污泥复合载体固定化微生物修复石油污染土壤  

Remediation of Petroleum-Contaminated Soil by Immobilized Microorganisms With Composite Carrier of Compost Sludge

作　　者：张秀霞[1] 李茹月 任立涵 熊康 李德 郝博宇 覃菲菲 李婧[1] 尹凌皓 傅晓升 ZHANG Xiuxia;LI Ruyue;REN Lihan;XIONG Kang;LI De;HAO Boyu;QIN Feifei;LI Jing;YIN Linghao;FU Xiaosheng(Department of Environmental and Safety Engineering,China University of Petroleum,Qingdao 266580,China;NO.1 Branch,Beijing Gas Group Co.,Ltd.,Beijing 100022,China)

机构地区：[1]中国石油大学(华东)环境与安全工程系,山东青岛266580 [2]北京市燃气集团有限责任公司第一分公司,北京100022

出　　处：《石油学报（石油加工）》2023年第3期544-551,共8页Acta Petrolei Sinica(Petroleum Processing Section)

基　　金：石油石化污染物控制与处理国家重点实验室开放基金项目(PPC2019021);中央高校基本科研业务费专项(22CX01004A-6);中国石油集团安全环保关键技术研究项目(2017-4613);中国石油科技创新基金研究项目(2018-5007-0605,2017-5007-0601)资助。

摘　　要：为了修复石油烃污染土壤并实现堆肥污泥的有效利用,以堆肥污泥和海藻酸钠作为复合载体,固定实验室筛选出的高效石油烃降解菌群制成复合载体固定化小球,用于修复石油烃污染土壤。考察了堆肥污泥质量分数、粒径大小对固定化小球性能的影响,通过扫描电镜观察堆肥污泥添加前后固定化小球中菌群的变化,比较了不同处理方法修复石油烃污染土壤的方案,确定了最佳修复方法。结果表明:当添加堆肥污泥的质量分数为1%时,固定化小球传质性能达到56%;当堆肥污泥的粒径从0.60 mm减小到0.25 mm时,石油烃的吸附率提高了17.14百分点;添加堆肥污泥制备的固定化小球处理土壤后,其内部可观察到的菌体数量明显增多,且降解后土壤中碳数≤15的石油烃占比增加,微生物群落丰度更高,是最佳的污染土壤修复方法。Using compost sludge and sodium alginate as composite carriers,and the high-efficiency petroleum hydrocarbon degrading bacteria group selected in a fixed laboratory,immobilized pellets were prepared for remediation of petroleum hydrocarbon contaminated soil.This aims to remediate petroleum hydrocarbon-contaminated soil and realize the effective utilization of compost sludge.The effect of mass fraction and particle size of compost sludge on the performance of the immobilized pellets was investigated;meanwhile,the schemes of remediating petroleum hydrocarbon contaminated soil through different approaches were compared based on the scanning electron microscopy observations of changes in microflora in the immobilized pellets before and after the addition of compost sludge,so as to determine the best remediation method.The results show that when the mass fraction of compost sludge is 1%,the mass transfer performance of the immobilized pellets can reach 56%;when the particle size of the compost sludge is reduced from 0.60 mm to 0.25 mm,the adsorption rate of petroleum hydrocarbons is increased by 17.14 percentage points.When the soil is treated with immobilized pellets prepared by adding compost sludge,the number of bacteria observed inside is increased significantly,the proportion of petroleum hydrocarbon carbon number≤15 in degraded soil is relatively large,and the abundance of microbial communities is higher,thus proving that it is the best remediation method.

关 键 词：石油污染土壤 固定化微生物 堆肥污泥 海藻酸钠 微生物修复 

分 类 号：X53[环境科学与工程—环境工程]