Pik3ip1通过抑制PI3K/AKT/mTOR信号通路促进牛MDSCs分化  

Pik3ip1 promoting differentiation of bovine skeletal muscle derived satellite cells(MDSCs)by inhibiting PI3K/AKT/mTOR signaling pathway

作　　者：严云勤[1] 滕怀昕 佟慧丽[1] 李树峰[1] 李爽[1] YAN Yunqin;TENG Huaixin;TONG Huili;LI Shufeng;LI Shuang(The Laboratory of Cell and Dvelepomental Biology,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学细胞与发育生物学实验室,哈尔滨150030

出　　处：《东北农业大学学报》2023年第4期55-63,共9页Journal of Northeast Agricultural University

基　　金：国家自然科学基金青年基金项目(31801150);东农学者“青年才俊”项目(20QC22);东农学者“学术骨干”项目(20XG10)。

摘　　要：PI3K相互作用蛋白1(Pik3ip1)为PI3K/AKT/mTOR信号通路负调控因子,其对牛MDSCs(Muscle derived satellite cells,MDSCs)分化研究较少。采用Western blot及免疫荧光,在牛MDSCs分化过程中检测Pik3ip1表达。构建Pik3ip1基因过表达及抑制载体,研究Pik3ip1对牛MDSCs分化及PI3K/AKT/mTOR信号通路活性的影响。结果表明,在牛MDSCs分化中Pik3ip1表达量逐渐升高;激活Pik3ip1表达后,细胞分化程度升高;抑制Pik3ip1表达则出现相反现象。Co-IP结果表明,在牛MDSCs分化过程中Pik3ip1与p110α相互作用。激活Pik3ip1抑制PI3K/AKT/mTOR信号通路活性。相反在抑制Pik3ip1表达后,PI3K/AKT/mTOR信号通路活性增强。综上所述,Pik3ip1通过抑制PI3K/AKT/mTOR信号通路促进牛MDSCs分化。Phosphatidylinositol-3-kinase Interacting Protein 1(Pik3ip1)has been characterized as a negative regulator of PI3K/AKT/mTOR pathway,which was rarely studied in bovine MDSCs.Pik3ip1 expression was assessed during differentiation of bovine MDSCs by Western blot and immunofluorescence.The overexpression and inhibition vectors of Pik3ip1 gene were constructed to study the effects of Pik3ip1 on the differentiation of bovine MDSCs and the activity of PI3K/AKT/mTOR signaling pathway.The results showed that expression of Pik3ip1 increased during differentiation of bovine MDSCs.After activation of Pik3ip1 expression,the degree of cell differentiation increased;inhibition of Pik3ip1 expression showed the opposite phenomenon.Co-IP results indicated that Pik3ip1 interacted with p110αduring the differentiation of bovine MDSCs.Activation of Pik3ip1 inhibited the PI3K/AKT/mTOR signaling pathway and enhanced cell differentiation.As expected,the opposite phenomenon was observed after suppressing the expression of Pik3ip1.In conclusion,Pik3ip1 could promote the differentiation of bovine MDSCs by inhibiting PI3K/AKT/mTOR signaling pathway.

关 键 词：细胞分化 MDSCS Pik3ip1 PI3K/AKT/MTOR 

分 类 号：S823[农业科学—畜牧学]