基于SCoT标记的胡椒属药用植物多样性分析  

Diversity Analysis of Piper Medicinal Plants Based on SCoT Markers

作　　者：零唯 杨丹 谭勇 黄鼎 林敬祯 明如宏 LING Wei;YANG Dan;TAN Yong(Institute of Pharmacy,Guangxi University of Chinese Medicine,Nanning,Guangxi 530000;Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine,Guangxi University of Chinese Medicine,Nanning,Guangxi 530200)

机构地区：[1]广西中医药大学药学院,广西南宁530000 [2]广西中医药大学广西壮瑶药重点实验室,广西南宁530200

出　　处：《安徽农业科学》2023年第8期182-186,202,共6页Journal of Anhui Agricultural Sciences

基　　金：广西自然科学基金面上项目(2018GXNSFAA281075);广西壮瑶药重点实验室项目(GXZYZZ2020-04);广西中医药大学研究生教育创新计划项目(YCSY2020094)。

摘　　要：采用正交设计优化胡椒属药用植物SCoT-PCR反应体系,并运用SCoT分子标记对7种胡椒属药用植物31份样品进行遗传多样性研究。结果表明,胡椒属药用植物SCoT-PCR最佳反应体系:总体积20μL,其中10×Buffer 2.0μL,dNTPs 0.20 mmol/L,Mg^(2+) 1.5 mmol/L,引物0.4μmol/L,模板DNA 100 ng,Taq聚合酶2.5 U。扩增总条带数为68条,其中多态性条带为64条,多态性比率为94.12%。31份胡椒属药用植物个体间聚类分析结果显示,31份胡椒属样品划分为2个类群,6个亚群。SCoT可适用于胡椒属药用植物遗传多样性研究。The SCoT-PCR reaction system of medicinal plants of Piper was optimized by orthogonal design,and the genetic diversity of 31 samples of 7 species of medicinal plants of Piper was studied by SCoT molecular markers.The results showed that the best SCoT-PCR reaction system of Piper was as follows:the total volume was 20μL,of which 10×buffer 2.0μL,dNTPs 0.20 mmol/L,Mg^(2+)1.5 mmol/L,primer 0.4μmmol/L,template DNA 100 ng,Taq polymerase 2.5 U.The total number of amplified bands was 68,of which 64 were polymorphic,with a polymorphism rate of 94.12%.The clustering results showed that 31 samples of Piper were divided into 2 groups and 6 subgroups.SCoT can be used to study the genetic diversity of medicinal plants of Piper.

关 键 词：胡椒属 SCoT 正交优化 遗传多样性 

分 类 号：Q949.732.3[生物学—植物学]