野豌豆属9份牧草资源的叶片宏观和微观形态差异  

Macroscopic and Microscopic Morphological Differences in the Leaves of Nine Forage Resoureces of Vicia L.

作　　者：侯燕红 段新慧[1] 栾旭辉 周凯 韩博[1] 姜华[1] 何承刚[1] HOU Yanhong;DUAN Xinhui;LUAN Xuhui;ZHOU Kai;HAN Bo;JIANG Hua;HE Chenggang(Faculty of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2023年第2期283-289,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省农业联合基金重点项目(202101BD070001-026);云南省产业创新人才项目;云南省重点研发计划课题(2018BB002-02);国家自然科学基金项目(31660682);云南省教育厅科学研究基金项目(2020Y174)。

摘　　要：【目的】对野豌豆属(Vicia L.)植物的叶片形态进行分析,为该属植物的分类鉴定提供形态学依据。【方法】以9份野豌豆属牧草资源为研究对象,分析其叶片的宏观和微观形态,并对所有指标进行主成分分析、相关性分析和聚类分析。【结果】9份野豌豆种间形态表现差异明显,宏观性状间的变异范围为0.00%~49.13%,遗传多样性指数范围为0.00~1.06。小叶形状与茎柔毛密度、小叶柔毛密度、气孔指数和气孔密度均呈极显著负相关(P<0.01);茎柔毛密度与小叶尖端形状和小叶柔毛密度呈极显著正相关(P<0.01),并且与托叶大小、气孔指数和气孔密度呈显著正相关(P<0.05);托叶大小与托叶有无锯齿呈极显著正相关(P<0.01);气孔指数与气孔密度呈极显著正相关(P<0.01)。主成分分析结果表明:茎柔毛密度、小叶柔毛密度、托叶大小、气孔指数和气孔密度的主成分累计贡献率达92.02%。【结论】9份野豌豆属牧草的叶片形态表现出丰富的差异性,且茎柔毛密度、小叶柔毛密度、托叶大小、气孔指数和气孔密度可作为分类鉴定的依据。[Purpose]To analyse the leaf morphology of Vicia L.,providing morphological basis for the classification and identification of Vicia.[Methods]Nine Vicia forage resources were taken as the research objects,the macro scopic and micro scopic morphology of leaves were analyzed,and principal component analysis,correlation analysis and cluster analysis were carried out on all indexes.[Results]There were obvious differences among nine Vicia species,with the variation range of 0.00%-49.13%,and the genetic diversity index range of 0.00-1.06.The shape of leaflets was extremely negatively correlated with stem pilose density,leaflet pilose density,stomatal index and stomatal density(P<0.01);the stem pilose density was extremely positively correlated with the shape of leaflet tip and the leaflet pilose density(P<0.01),and positively correlated with stipule size,stomatal index and stomatal density(P<0.05);stipule size was extremely positively correlated with the serration of stipules(P<0.01);stomatal index was extremely positively correlated with stomatal density(P<0.01).The results of principal component analysis showed that the cumulative contribution rate of the stem pilose density,leaflet pilose density,stipule size,stomatal index and stomatal density reached 92.02%.[Conclusion]The leaf morphology of nine Vicia species shows rich differences,and the main sources of the differences are stem pilose density,leaflet pilose density,stipule size,stomatal index and stomatal density,which can be used as the basis for classification and identification.

关 键 词：野豌豆属 叶片形态特征 主成分分析 聚类分析 

分 类 号：S541.901[农业科学—作物学]