mPEG链长及分散性对mPEG-TPE胶束行为影响  

Effects of chain length and polydispersity of mPEG on physicochemical properties of mPEG-TPE micelles

作　　者：郝祖杭 袁于民 HAO Zuhang;YUAN Yumin(School of Materials Science and Engineering,Zhejiang Sci-Tech University,Hangzhou 310018,China;Institute of Smart Biomedical Materials,Zhejiang Sci-Tech University,Hangzhou 310018,China;Biomatrik Inc.,Jiaxing 314001,China)

机构地区：[1]浙江理工大学材料科学与工程学院,杭州310018 [2]浙江理工大学智能生物材料研究所,杭州310018 [3]浙江博美生物技术有限公司,浙江嘉兴314001

出　　处：《浙江理工大学学报（自然科学版）》2023年第3期365-373,共9页Journal of Zhejiang Sci-Tech University(Natural Sciences)

摘　　要：为研究聚乙二醇单甲醚(mPEG)链长及分散性对其胶束的理化性质及不同介质下稳定性的影响,采用具有聚集发光特性的四苯乙烯(Tetraphenylethylene,TPE)为憎水段,合成了3种不同链长的单分散mPEGn-TPE酯(n=15,23,35),以及1种多分散mPEG1000-TPE酯的双亲分子。探讨了mPEG链长及分散性的差异对mPEG-TPE胶束的临界胶束浓度(Critical micelle concentration,CMC)、粒径、荧光强度、水解速度及在牛血清白蛋白(BSA)溶液中稳定性的影响。结果表明:所合成4种mPEG-TPE化合物均在水溶液中形成自组装胶束并展现出AIE特征,胶束的尺寸、CMC值及在磷酸盐缓冲液(PBS)中的水解速度,随着PEG链长的增长而增加,但荧光强度减弱,这说明胶束内核TPE之间的相互作用减弱;在BSA介质下,保持胶束稳定的时间随PEG链增长而增加,与mPEG1000-TPE胶束对比,分子量相当的单分散mPEG23-TPE胶束在BSA溶液中能保持更长时间的稳定,展示了单分散的优势。因此,纳米载体系统在临床应用时很有必要采用单分散的PEG,以获得更好的药物递送效果。To provide a greater insight to the effects of polyethylene glycol(PEG)length and the molecular weight dispers ity on the physical properties of methoxy PEG-tetraphenyl ethylene(mPEG-TPE)self-assembled micelles,three monodisperse mPEGn-TPEs(n=15,23 and 35)and one polydisperse counterpart mPEG1000-TPE were synthesized,utilizing the hydrophobic nature of,and more importantly,aggregation induced emission(AIE)characteristics of TPE moiety.Critical micelle concentration(CMC),micelle size,fluorescent intensity,stability in both PBS and BSA solutions were investigated.Results indicate that the CMC,sizes of micelle,and stability in BSA solution increase,while fluorescent intensity,stability in PBS solution decrease,with increasing the repeat unit of n.Interestingly,the dispersity of PEG length has a significant impact on their micelle stability in BSA solution.Monodisperse mPEG23-TPE has a much better stability than its polydisperse mPEG1000-TPE counterpart in BSA solution,suggesting that monodispersity of PEG could be a preferred choice when a nano-formulation is to be developed for a drug delivery application.

关 键 词：单分散聚乙二醇 四苯乙烯 聚集诱导发光 胶束 粒径 抗蛋白吸附 

分 类 号：TQ432.2[化学工程]