Two lineages of bivalve transmissible neoplasia affect the blue mussel Mytilus trossulus Gould in the subarctic Sea of Okhotsk  

作　　者：Maria Skazina Nelly Odintsova Mariia Maiorova Lidia Frolova Irina Dolganova Kira Regel Petr Strelkov 

机构地区：[1]Department of Applied Ecology,St Petersburg State University,St.Petersburg 199034,Russia [2]National Scientific Center of Marine Biology,Far Eastern Branch of the Russian Academy of Sciences,Vladivostok 690041,Russia [3]Institute of the Biological Problems of the North,Far Eastern Branch of the Russian Academy of Sciences,Magadan 685000,Russia [4]Laboratory of Monitoring and Conservation of Natural Arctic Ecosystems,Murmansk Arctic State University,Murmansk 183038,Russia

出　　处：《Current Zoology》2023年第1期91-102,共12页动物学报（英文版）

基　　金：supported by the Russian Science Foundation,Grant Number 19-74-20024.

摘　　要：There are increasing findings of the bivalve transmissible neoplasia derived from the Pacific mussel Mytilus trossulus(MtrBTN)in populations of different Mytilus species worldwide.The Subarctic is an area where this disease has not yet been sought despite the fact that Mytilus spp.are widespread there,and M.trossulus itself is a boreal species.We used flow cytometry of the hemolymph,hemocytology,and histology to diagnose disseminated neoplasia in a sample of M.trossulus from Magadan in the subarctic Sea of Okhotsk.Neoplasia was identified in 11 of 214 mussels studied.Using mtDNA COI sequencing,we revealed genotypes identical or nearly identical to known MtrBTN ones in the hemolymph of most of the diseased mussels.Both MtrBTN evolutionary lineages have been identified,the widespread MtrBTN2,and MtrBTN1,so far only known from M.trossulus in British Columbia on the other side of the Pacific from Magadan.In addition,MtrBTN2 was represented by 2 common diverged mtDNA haplolineages.These conclusions were confirmed for selected cancerous mussels by molecular cloning of COI and additional nuclear and mtDNA genes.On the background of high genetic diversity,different cancers were similar in terms of ploidy(range 4.0–5.8 n)and nuclear-to-cell ratio.Our study provides the first description of neoplasia and MtrBTN in mussels from the Sea of Okhotsk and from the Subarctic,of both MtrBTN1 and MtrBTN2 in the same mussel population,and the first direct comparison between these transmissible cancers.

关 键 词：disseminated neoplasia molecular identification MYTILUS Pacific subarctic transmissible cancer 

分 类 号：Q95[生物学—动物学]