Correlative light-electron microscopy using small gold nanoparticles as single probes  

作　　者：Iestyn Pope Hugh Tanner Francesco Masia Lukas Payne Kenton Paul Arkilll Judith Mantell Wolfgang Langbein Paola Borri Paul Verkade 

机构地区：[1]School of Biosciences,Cardiff University,Museum Avenue,Cardiff CF103AX,UK [2]School of Biochemistry,University of Bristol,University Walk,Bristol,UK [3]Department of Chemistry,Umea University,Umea 90187,Sweden [4]School of Physics and Astronomy,Cardiff University,The Parade,Cardiff CF243AA,UK [5]School of Medicine,University of Nottingham,NottinghamNG72RD,UK

出　　处：《Light(Science & Applications)》2023年第4期657-670,共14页光（科学与应用)（英文版）

基　　金：funded by the UK EPSRC Research Council(Grants EP/l005072/1,EP/l016260/1,EP/L001470/1,EP/M028313/1);the UKBBSRC Research Council(Grants BBL014181/1,BB/M001969/1).

摘　　要：Correlative light-electron microscopy(CLEM)requires the availability of robust probes which are visible both in light and electron microscopy.Here we demonstrate a CLEM approach using small gold nanoparticles as a single probe.Individual gold nanoparticles bound to the epidermal growth factor protein were located with nanometric precision background-free in human cancer cells by light microscopy using resonant four-wave mixing(FWM),and were correlatively mapped with high accuracy to the corresponding transmission electron microscopy images.We used nanoparticles of 10 nm and 5 nm radius,and show a correlation accuracy below 60 nm over an area larger than 10 pm size,without the need for additional fiducial markers.Correlation accuracy was improved to below 40 nm by reducing systematic errors,while the localisation precision is below 10 nm.Polarisation-resolved FWM correlates with nanoparticle shapes,promising for multiplexing by shape recognition in future applications.Owing to the photostability of gold nanoparticles and the applicability of FWM microscopy to living cells,FWM-CLEM opens up a powerful alternative to fluorescence-based methods.

关 键 词：LIGHT RADIUS RESONANT 

分 类 号：O43[机械工程—光学工程]