Ethanol Extract of Glycyrrhiza uralensis Fisch:Antidiarrheal Activity in Mice and Contraction Effect in Isolated Rabbit Jejunum  

作　　者：WEN Jing ZHANG Jian-wu LYU Yuan-xia ZHANG Hui DENG Kai-xi CHEN Hong-xue WEI Ying 

机构地区：[1]Department of Pharmacy,North Sichuan Medical College,Nanchong,Sichuan Province 637100,China

出　　处：《Chinese Journal of Integrative Medicine》2023年第4期325-332,共8页中国结合医学杂志（英文版）

基　　金：Supported by Innovation and Entrepreneurship Training Program for College Students in Sichuan Province(No.S201910634082,202110634018);Nanchong Government-Municipal Colleges and Universities Cooperative Scientific Research Project(No.19SXHZ0231);Doctor Initiation Fund of North Sichuan Medical College(No.CBY19-QD07)。

摘　　要：Objective:To evaluate the antidiarrheal effect of ethanol extract of Glycyrrhiza uralensis Fisch root(GFR)in vivo and jejunal contraction in vitro.Methods:In vivo,50 mice were divided into negative control,positive control(verapamil),low-,medium-and high-dose GFR(250,500,1,000 mg/kg)groups by a random number table,10 mice in each group.The antidiarrheal activity was evaluated in castor oil-induced diarrhea mice model by evacuation index(El).In vitro,the effects of GFR(0.01,0.03,0.1,0.3,1,3,and 10 g/L)on the spontaneous contraction of isolated smooth muscle of rabbit jejunum and contraction of pretreated by Acetylcholine(ACh,10μmol/L)and KCl(60 mmol/L)were observed for 200 s.In addition,CaCl_(2)was accumulated to further study its mechanism after pretreating jejunal smooth muscle with GFR(1 and 3 g/L)or verapamil(0.03 and 0.1μmol/L)in a Ca^(2+)-free-high-K+solution containing ethylene diamine tetraacetic acid(EDTA).Results:GFR(500 and 1,000 mg/kg)significantly reduced El in castor oil-induced diarrhea model mice(P<0.01).Meanwhile,GFR(0.01,0.03,0.1,0.3,1,3,and 10 g/L)inhibited the spontaneous contraction of rabbit jejunum(P<0.05 or P<0.01).Contraction of jejunums samples pretreated by ACh and KCI with 50% effective concentration(EC50)values was 1.05(0.71-1.24),0.34(0.29-0.41)and 0.15(0.11-0.20)g/L,respectively.In addition,GFR moved the concentration-effect curve of CaCl_(2)down to the right,showing a similar effect to verapamil.Conclusions:GFR can effectively reduce diarrhea and inhibit intestinal contraction,and these antidiarrheal effects may be based on blocking L-type Ca^(2+) channels and muscarinic receptors.

关 键 词：Glycyrrhiza uralensis Fisch ANTIDIARRHEAL spasmolysis Chinese medicine 

分 类 号：R285.5[医药卫生—中药学]