健康妊娠妇女不同孕期血清补体因子的变化及其参考区间的建立  

Changed levels of complement components in serum of healthy pregnant women during different trimesters and establishment of reference interval

作　　者：矫祯浩 马利娟 顾珣可 王永清[2] 贾珂珂[1] JIAO Zhenhao;MA Lijuan;GU Xunke;WANG Yongqing;JIA Keke(Department of Laboratory Medicine,Peking University Third Hospital,Beijing 100191,China;Department of Obsterics,Peking University Third Hospital,Beijing 100191,China)

机构地区：[1]北京大学第三医院检验科,北京100191 [2]北京大学第三医院妇产科,北京100191

出　　处：《临床检验杂志》2023年第2期154-157,共4页Chinese Journal of Clinical Laboratory Science

基　　金：国家自然科学基金面上项目(62071007)。

摘　　要：目的探讨健康妊娠妇女血清补体C1q、B因子、H因子以及C3、C4在不同孕期的变化,并建立其参考区间。方法选择398例健康妊娠妇女,按照孕周将其分为孕早期、孕中期和孕晚期,并选取120例健康非妊娠妇女作为健康未孕组。采用免疫透射比浊法测定血清C3、C4、H因子、B因子、补体C1q的水平。比较各组之间补体因子的差异,依据美国临床和实验室标准化协会(CLSI)EP28-A3c要求,以非参数法建立不同孕期血清C1q等补体因子的95%参考区间。结果血清补体C1q和C4水平在各孕期之间差异无统计学意义,妊娠期血清C1q的参考区间为135~254 mg/L,补体C4为131~387 mg/L。妊娠早期、中期及晚期其他补体因子参考区间:B因子分别为255~397 mg/L、294~493 mg/L、312~615 mg/L,H因子分别为228~433 mg/L、276~446 mg/L、299~466 mg/L,补体C3分别是889~1557 mg/L、1081~1664 mg/L、1139~1822 mg/L。结论相比健康非妊娠妇女,健康妊娠妇女的血清补体C1q水平无显著变化,而B因子、H因子、补体C3、补体C4水平均有不同程度的升高。本研究建立了健康妊娠妇女血清C1q、C4的参考区间以及不同孕期血清B因子、H因子、C3的参考区间。Objective To investigate the changed levels of complement C1q,factor B,factor H,C3 and C4 in the serum of healthy pregnant women during different trimesters and establish their reference intervals.Methods A total of 398 healthy pregnant women were enrolled in this study and divided into 3 groups:early pregnancy,middle pregnancy and late pregnancy according to their gestational age,and 120 healthy non-pregnant women were selected as the control group.The serum levels of complement component C3,C4,factor H,factor B and C1q were determined by immunotransmission turbidimetry.In accordance with the requirements of the Clinical and Laboratory Standards Institute(CLSI)EP28-A3c,the differences of complement component levels among each group were analyzed by the nonparametric 95%percentile method to calculate the reference interval of C1q and other complement components in different trimesters.Results There were no significant difference for the levels of C1q and C4 during different trimesters.The reference interval of serum C1q level in pregnant women was 135 to 254 mg/L and C4 level was 131 to 387 mg/L.The reference intervals of other complement components in early,middle and late pregnancy group were as follows:255 to 397 mg/L,294 to 493 mg/L and 312 to 615 mg/L for factor B,respectively,228 to 433 mg/L,276 to 446 mg/L and 299 to 466 mg/L for factor H,889 to 1557 mg/L,1081 to 1664 mg/L and 1139 to 1822 mg/L for C3,respectively.Conclusion Compared to the non-pregnant control group,the level of C1q in serum of healthy pregnant women was slightly decreased,while the levels of factor B,factor H,C3 and C4 increased to varying degrees.The reference intervals of serum C1q and C4 in healthy pregnant women and those of factor B,factor H and C3 in the different trimesters were established.

关 键 词：妊娠 血清补体C1q C3 C4 H因子 B因子 参考区间 

分 类 号：R446.6[医药卫生—诊断学]