支气管哮喘患儿血清Rac1水平变化及临床意义  

The changes and clinical significance of serum Rac1 levels in children with bronchial asthma

作　　者：王亚南 戴倩倩 茹凉[1] WANG Yanan;DAI Qianqian;RU Liang(Department of Pediatrics of the First Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang 830000,China)

机构地区：[1]新疆医科大学第一附属医院儿科,新疆乌鲁木齐830000

出　　处：《中华全科医学》2023年第5期736-739,共4页Chinese Journal of General Practice

基　　金：新疆维吾尔自治区自然科学基金项目(2019D01C310)。

摘　　要：目的通过检测支气管哮喘患儿血清Ras相关的C3肉毒素底物1(Rac1)水平变化,探讨其与患儿病情严重程度及预后的关系。方法选择2019年1月—2022年1月新疆医科大学第一附属医院收治的支气管哮喘患儿150例为观察组,根据病情严重程度分为轻度组(49例)、中度组(59例)、重度组(42例),以健康儿童80例为对照组,采用RT-PCR法检测受检者血清单个核细胞Rac1 mRNA水平,采用Pearson分析Rac1 mRNA与患儿气道炎症反应及肺功能的相关性,采用logistic回归分析研究支气管哮喘患儿预后的危险因素。结果轻度组(0.14±0.03)、中度组(0.07±0.02)、重度组(0.06±0.03)的Rac1 mRNA均低于对照组(0.16±0.03),t=3.675、19.990、17.493,均P<0.05;Rac1 mRNA与哮喘患儿白介素-25(IL-25)、白介素-33(IL-33)、呼出气一氧化氮(FeNO)、免疫球蛋白E(IgE)呈明显负相关关系(r=-0.620、-0.691、-0.660、-0.634,均P<0.01),与第1秒用力呼气流量(FEV 1)、FEV 1/用力肺活量(FVC)、呼气流量峰值(PEF)呈明显正相关关系(r=0.648、0.582、0.522,均P<0.01)。Rac1 mRNA高水平为影响哮喘患儿预后的保护因素(OR=0.753,95%CI:0.647~0.877,P<0.001)。结论支气管哮喘患儿血清Rac1水平较低,与气道炎症反应、肺功能下降相关。Objective Through detecting the changes of serum Ras-related C3 botulinum toxin substrate 1(Rac1)levels in children with bronchial asthma,the relationship between them and the severity and prognosis of the children's disease was investigated.Methods One hundred and fifty children with bronchial asthma admitted to the First Affiliated Hospital of Xinjiang Medical University from January 2019 to January 2022 were selected as the observation group and divided into mild(49 cases),moderate(59 cases)and severe(42 cases)groups according to the severity of disease.Another 80 healthy children were selected as the control group.The Rac1 mRNA of serum monocyte cells was detected by RT-PCR.The correlation between Rac1 mRNA and the airway inflammatory response and lung function of the children was analyzed by Pearson,and the risk factors for the prognosis of children with bronchial asthma were analyzed by logistic regression.Results The Rac1 mRNA was lower in the mild(0.14±0.03),moderate(0.07±0.02)and severe(0.06±0.03)groups than that in the control group(0.16±0.03),t=3.675,19.990,17.493,all P<0.05;Rac1 mRNA was significantly negatively correlated with IL-25,IL-33,fractional exhaled nitric oxide(FeNO),IgE(r=-0.620,-0.691,-0.660,-0.634,all P<0.01)and positively correlated with the forced expiratory volume in one second(FEV 1),FEV 1/forced vital capacity(FVC),peak expiratory flow(PEF)in children with asthma(r=0.648,0.582,0.522,all P<0.01).High level of Rac1 mRNA was a protective factor affecting the prognosis of children with asthma(OR=0.753,95%CI:0.647-0.877,P<0.001).Conclusion Lower serum Rac1 levels in children with bronchial asthma are associated with airway inflammatory response and decreased lung function.

关 键 词：支气管哮喘 气道炎症反应 肺功能 C3肉毒素底物1 相关性 

分 类 号：R562.25[医药卫生—呼吸系统] R725.6[医药卫生—内科学]