HPLC法同时测定生骨补肾胶囊中两种活性成分含量研究  

Simultaneous Determination of Two Constituents in Shenggu Bushen Capsules by HPLC

作　　者：罗黎霞 谢嘉驰 毛文点 朱金 罗纯清 杨宁 Luo Lixia;Xie Jiachi;Mao Wendian;Zhu Jin;Luo Chunqing;Yang Ning(Department of Pharmacy,Xiangtan Hospital of Traditional Chinese Medicine,Xiangtan 411100,China)

机构地区：[1]湘潭市中医医院药学部,湖南湘潭411100

出　　处：《山东化工》2023年第5期176-177,181,共3页Shandong Chemical Industry

基　　金：2020年湘潭市科技计划项目(SF-YB20201025)。

摘　　要：目的:同时建立生骨补肾胶囊中两种活性成分的测定方法,对该制剂的质量进行评价和控制。方法:采用HPLC法,Shim-pack VP-ODS色谱柱(4.6 mm×150 mm, 5μm),梯度洗脱:甲醇(A)-水(B)为流动相,流速0.8 mL/min,检测波长丹参酮IIA 270 nm、欧前胡素320 nm;柱温25℃,进样量20μL,制备3批供试品对丹参酮IIA、欧前胡素进行指标成分检测。结果:丹参酮IIA在3.75~200μg/mL线性关系良好(r=0.999 7),欧前胡素在3~120μg/mL线性关系良好(r=0.999 8)。结论:本研究所用方法简便快速、准确可靠,可作为生骨补肾胶囊的质量控制参考依据。Objective:To establish a method for the determination of two active ingredients in Shenggu Bushen capsule,and to evaluate and control the quality of the preparation.Methods:HPLC was performed on A Shim-pack VP-ODS column(4.6 mm×150 mm,5μm)with gradient elution:methanol(A)-water(B)as mobile phase at a flow rate of 0.8 mL/min.The detection wavelengths were Tanshinone IIA 270 nm and Imperatorin 320 nm.The column temperature was 25℃,and the injection volume was 20μL.Three batches of samples were prepared to detect the index components of tanshinone IIA and imperatorin.Results:Tanshinone IIA had a good linear relationship between 3.75~200μg/mL(r=0.9997),and Imperatin had a good linear relationship between 3~120μg/mL(r=0.9998).Conclusion:The method used in this study is simple,rapid,accurate and reliable,which can be used as reference for quality control of Shenggu Bushen capsule.

关 键 词：HPLC 生骨补肾胶囊 同时测定 丹参酮IIA 欧前胡素 

分 类 号：O657.7[理学—分析化学]