壳聚糖修饰载鲎血蛋白-白蛋白微球的制备及其活性研究  

Preparation and Activity of Chitosan-modif ied Limulus Lysate Proteinalbumin Microspheres

作　　者：曾紫怡 蔡鹰 刘佳华 廖思丽 张国光 ZENG Zi-yi;CAI Ying;LIU Jia-hua;LIAO Si-li;ZHANG Guo-guang(School of Chemistry and Environment,Guangdong Ocean University,Zhanjiang 524088,China)

机构地区：[1]广东海洋大学化学与环境学院,广东湛江524088

出　　处：《化工管理》2023年第14期62-65,共4页Chemical Engineering Management

基　　金：国家级大学生创新创业训练计划项目“壳聚糖修饰载鲎血蛋白-白蛋白微球的制备及其活性探究”(202110566032)。

摘　　要：文章通过分析壳聚糖修饰的牛血清白蛋白为载体制备包载鲎血蛋白的微球,考察制备微球的最佳工艺和活性。载药微球粒径大小较为均一,粒径范围为(285±54)nm。Zeta电位值为(-42.69±3.69)mV,包封率为(83.075±4.86)%,载药量为(34.25±2.41)%,在人工血液(pH=7.4)、肠液(pH=6.8)、胃液(pH=1.2)中24 h的累积释放量分别为55.63%、55.25%、48.75%。载药微球生物相容性高,稳定性好,药物释放具有明显的缓释效果。Chitosan-modif ied bovine serum albumin(BSA)was used as the carrier to prepare the microspheres containing tachypleus amebocyte lysate(TAL)blood protein.The size of drug-loaded microspheres is relatively uniform,and the particle size range is(285±54)nm.Zeta potential value was(-42.69±3.69)mV,entrapment rate was(83.075±4.86)%,drug loading was(34.25±2.41)%,and cumulative release in artif icial blood(pH=7.4),intestinal juice(pH=6.8),and gastric juice(pH=1.2)for 24 hours was 55.63%,55.25%,and 48.75%,respectively.The drug-loaded microspheres have high biocompatibility and good stability,and the drug release has obvious slow release effect.

关 键 词：壳聚糖 牛血清白蛋白 鲎血蛋白 微球 

分 类 号：Q5[生物学—生物化学]