Genome assembly of the Brassicaceae diploid Orychophragmus violaceus reveals complex whole-genome duplication and evolution of dihydroxy fatty acid metabolism  

作　　者：Fan Huang Peng Chen Xinyu Tang Ting Zhong Taihua Yang Chinedu Charles Nwafor Chao Yang Xianhong Ge Hong An Zaiyun Li Edgar B.Cahoon Chunyu Zhang 

机构地区：[1]National Key Lab of Crop Genetic Improvement and College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,China [2]Center for Plant Science Innovation,University of Nebraska-Lincoln,Lincoln,NE,USA [3]Department of Biochemistry,University of Nebraska-Lincoln,Lincoln,NE,USA [4]Bioinformatics and Analytics Core,University of Missouri-Columbia,Columbia,MO,USA

出　　处：《Plant Communications》2023年第2期93-106,共14页植物通讯（英文）

基　　金：supported by the National Natural Science Foundation of China(U20A2034 and 31871659);the China Agriculture Research System(CARS-12)(to C.Z.);E.B.C.was supported by funding from the National Science Foundation(Plant Genome IOS-13-39385).

摘　　要：Orychophragmus violaceus is a Brassicaceae species widely cultivated in China,particularly as a winter cover crop in northern China because of its low-temperature tolerance and low water demand.Recently,O.violaceus has also been cultivated as a potential industrial oilseed crop because of its abundant 24-carbon dihydroxy fatty acids(diOH-FAs),which contribute to superior high-temperature lubricant properties.In this study,we performed de novo assembly of the O.violaceus genome.Whole-genome synteny analysis of the genomes of its relatives demonstrated that O.violaceus is a diploid that has undergone an extrawhole-genome duplication(WGD)after the Brassicaceae-specific a-WGD event,with a basic chromosome number of x=12.Formation of diOH-FAs is hypothesized to have occurred after the WGD event.Based on the genome and the transcriptome data from multiple stages of seed development,we predicted that OvDGAT1-1 and OvDGAT1-2 are candidate genes for the regulation of diOH-FA storage in O.violaceus seeds.These results may greatly facilitate the development of heat-tolerant and eco-friendly plant-based lubricants using O.violaceus seed oil and improve our understanding of the genomic evolution of Brassicaceae.

关 键 词：Orychophragmus violaceus genome evolution dihydroxy fatty acids polyestolides lubricant oil OILSEED Brassicaceae 

分 类 号：Q94[生物学—植物学]