An activated form of NB-ARC protein RLS1 functions with cysteine-rich receptor-like protein RMC to trigger cell death in rice  

作　　者：Yiqin Wang Zhenfeng Teng Hua Li Wei Wang Fan Xu Kai Sun Jinfang Chu Yangwen Qian Gary JLoake Chengcai Chu Jiuyou Tang 

机构地区：[1]State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,the Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100101,China [2]Institute of Genetics and Developmental Biology and National Center for Plant Gene Research(Beijing),Chinese Academy of Sciences,Beijing 100101,China [3]Biogle Genome Editing Center,Changzhou 213125,China [4]Institute of Molecular Plant Sciences,School of Biological Sciences,University of Edinburgh,Edinburgh EH93BF,UK [5]Guangdong Laboratory for Lingnan Modern Agriculture,State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,College of Agriculture,South China Agricultural University,Guangzhou 510642,China

出　　处：《Plant Communications》2023年第2期151-166,共16页植物通讯（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(grant numbers 31571248,31430063,and 31871586).

摘　　要：A key event that follows pathogen recognition by a resistance(R)protein containing an NB-ARC(nucleotide-binding adaptor shared by Apaf-1,R proteins,and Ced-4)domain is hypersensitive response(HR)-type cell death accompanied by accumulation of reactive oxygen species and nitric oxide.However,the integral mechanisms that underlie this process remain relatively opaque.Here,we show that a gain-offunction mutation in the NB-ARC protein RLS1(Rapid Leaf Senescence 1)triggers high-light-dependent HR-like cell death in rice.The RLS1-mediated defense response is largely independent of salicylic acid accumulation,NPR1(Nonexpressor of Pathogenesis-Related Gene 1)activity,and RAR1(Required for Mla12 Resistance 1)function.A screen for suppressors of RLS1 activation identified RMC(Root Meander Curling)as essential for the RLS1-activated defense response.RMC encodes a cysteine-rich receptor-like secreted protein(CRRSP)and functions as an RLS1-binding partner.Intriguingly,their co-expression resulted in a change in the pattern of subcellular localization and was sufficient to trigger cell death accompanied by a decrease in the activity of the antioxidant enzyme APX1.Collectively,our findings reveal an NBARC-CRRSP signaling module that modulates oxidative state,the cell death process,and associated immunity responses in rice.

关 键 词：hypersensitive response NB-ARC protein cysteine-rich receptor-like secreted protein disease resistance antioxidant enzymes 

分 类 号：Q94[生物学—植物学]