Engineering crop Phytophthora resistance by targeting pathogen-derived PI3P for enhanced catabolism  

作　　者：Kun Yang Qiang Yan Yi Wang Wenyi Zhu Xiaodan Wang Xiaobo Li Hao Peng Yang Zhou Maofeng Jing Daolong Dou 

机构地区：[1]Key Laboratory of Plant Immunity,College of Plant Protection,Academy for Advanced Interdisciplinary Studies,Nanjing Agricultural University,Nanjing 210095,China [2]Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanjing 210095,China [3]College of Plant Protection,China Agricultural University,Beijing 100091,China [4]Crops Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Crop Genetic Improvement,Guangdong,Guangzhou 510640,China [5]Department of Plant Pathology,Washington State University,Pullman,WA 99164,USA

出　　处：《Plant Communications》2023年第2期167-178,共12页植物通讯（英文）

基　　金：supported by the National Natural Science Foundation of China,China(32072507,32272495,and 31721004);the Natural Science Foundation of Jiangsu Province,China(BK20220147).

摘　　要：Phytophthora pathogens lead to numerous economically damaging plant diseases worldwide,including potato late blight caused by P.infestans and soybean root rot caused by P.sojae.Our previous work showed that Phytophthora pathogens may generate abundant phosphatidylinositol 3-phosphate(PI3P)to promote infection via direct association with RxLR effectors.Here,we designed a disease control strategy for metabolizing pathogen-derived PI3P by expressing secreted Arabidopsis thaliana phosphatidylinositol-4-phosphate 5-kinase 1(AtPIP5K1),which can phosphorylate PI3P to PI(3,4)P2.We fused AtPIP5K1 with the soybean PR1a signal peptide(SP-PIP5K1)to enable its secretion into the plant apoplast.Transgenic soybean and potato plants expressing SP-PIP5K1 showed substantially enhanced resistance to various P.sojae and P.infestans isolates,respectively.SP-PIP5K1 significantly reduced PI3P accumulation during P.sojae and soybean interaction.Knockout or inhibition of PI3 kinases(PI3Ks)in P.sojae compromised the resistance mediated by SP-PIP5K1,indicating that SP-PIP5K1 action requires a supply of pathogen-derived PI3P.Furthermore,we revealed that SP-PIP5K1 can interfere with the action of P.sojae mediated by the RxLR effector Avr1k.This novel disease control strategy has the potential to confer durable broad-spectrum Phytophthora resistance in plants through a clear mechanism in which catabolism of PI3P interferes with RxLR effector actions.

关 键 词：AtPIP5K1 Phytophthora resistance PI3P RxLR effector transgenic plant 

分 类 号：S43[农业科学—农业昆虫与害虫防治]