Orthologous genesPm12andPm21from twowild relatives of wheat show evolutionary conservation but divergent powdery mildew resistance  

作　　者：Shanying Zhu Cheng Liu Shuangjun Gong Zhaozhao Chen Rong Chen Tianlei Liu Renkang Liu Haonan Du Rui Guo Genying Li Miaomiao Li Renchun Fan Zhiyong Liu Qian-Hua Shen Anli Gao Pengtao Ma Huagang He 

机构地区：[1]School of Environment and Safety Engineering,Jiangsu University,Zhenjiang 212013,China [2]School of Life Sciences,Jiangsu University,Zhenjiang 212013,China [3]Crop Research Institution,Shandong Academy of Agricultural Sciences,Jinan 250100,China [4]Institute of Plant Protection and Soil Science,Hubei Academy of Agricultural Sciences,Wuhan 430064,China [5]School of Life Sciences,Henan University,Kaifeng 475004,China [6]State Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics and Developmental Biology,The Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100101,China [7]College of Life Sciences,Yantai University,Yantai 264005,China

出　　处：《Plant Communications》2023年第2期236-248,共13页植物通讯（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(32171990,32072053,31971874,31872009,and U1604116);the Key Research and Development Program of Zhenjiang(NY2021001);the State Key Laboratory of Plant Cell and Chromosome Engineering(PCCE-KF-2021-05,PCCE-KF-2022-07);the State Key Laboratory of Crop Biology in Shandong Agricultural University(2021KF01);the Taishan Scholars Project(tsqn201812123);the Key Research and Development Program of Yantai(2019YT06000470).

摘　　要：Wheat powdery mildew,caused by Blumeria graminis f.sp.tritici(Bgt),is a devastating disease that threatens wheat production worldwide.Pm12,which originated from Aegilops speltoides,a wild relative of wheat,confers strong resistance to powdery mildew and therefore has potential use in wheat breeding.Using susceptible mutants induced by gamma irradiation,we physically mapped and isolated Pm12 and showed it to be orthologous to Pm21 from Dasypyrum villosum,also a wild relative of wheat.The resistance function of Pm12 was validated via ethyl methanesulfonatemutagenesis,virus-induced gene silencing,and stable genetic transformation.Evolutionary analysis indicates that the Pm12/Pm21 loci in wheat species are relatively conserved but dynamic.Here,we demonstrated that the two orthologous genes,Pm12 and Pm21,possess differential resistance against the same set of Bgt isolates.Overexpression of the coiledcoil domains of both PM12 and PM21 induces cell death in Nicotiana benthamiana leaves.However,their full-length forms display different cell death-inducing activities caused by their distinct intramolecular interactions.Cloning of Pm12 will facilitate its application in wheat breeding programs.This study also gives new insight into two orthologous resistance genes,Pm12 and Pm21,which show different race specificities and intramolecular interaction patterns.

关 键 词：Pm12 PM21 powdery mildew resistance evolutionary conservation race specificity intramolecular interaction 

分 类 号：S435[农业科学—农业昆虫与害虫防治]