基于UPLC-triple-TOF-MS/MS比较桑树桑黄和瓦尼桑黄发酵产物的抗氧化活性  

A comparative study on antioxidant activities of fermentation products of Sanghuangporus sanghuang and S.vaninii based on UPLC-triple-TOF-MS

作　　者：吕国英[1] 宋婷婷[1] 张洋洋 蔡为明[1] 张作法[1] LÜGuoying;SONG Tingting;ZHANG Yangyang;CAI Weiming;ZHANG Zuofa(Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,Zhejiang,China)

机构地区：[1]浙江省农业科学院园艺研究所,浙江杭州310021

出　　处：《菌物学报》2023年第4期939-948,共10页Mycosystema

基　　金：浙江省农业新品种选育重大科技专项(2021C02073)。

摘　　要：桑黄Sanghuangporus spp.是一类具有广泛的药理活性的大型真菌,其卓越的抗氧化活性备受关注。本研究比较了桑树桑黄Sanghuangporus sanghuang(桑黄)和瓦尼桑黄S.vaninii(杨黄)发酵产物乙醇提取物的抗氧化活性,并分析它们化学成分的差异。实验以1,1-二苯基-2-三硝基苯肼(DPPH)清除率、羟基自由基引起的DNA损伤的保护作用和L929细胞抗衰老实验作为指标比较其抗氧化活性差异。结果表明,桑树桑黄和瓦尼桑黄发酵产物提取物均表现出良好的抗氧化性,而且桑树桑黄发酵物的抗氧化能力显著高于瓦尼桑黄。通过超高效液相色谱串联三重四级杆飞行时间质谱法(UPLC-triple-TOF-MS)从桑黄中共鉴定出11种多酚类物质,瓦尼桑黄发酵产物成分相对简单。Sanguangporus spp.are a group of fungi with a wide range of pharmacological activities,and their excellent antioxidant activity has attracted people’s extensive attention.Antioxidant activities and chemical components of ethanol extracts of fermentation products from Sanghuangporus sanghuang and S.vaninii were compared.The scavenging rate of 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH),the protective effect of DNA damage caused by hydroxyl radical and L929 cell anti-ageing effect were used to compare the differences of antioxidant activity.The results showed that the extracts of fermentation products from S.sanghuang and S.vaninii both showed effective antioxidant activities,and the antioxidant activity of S.sanghuang fermented product was significantly stronger than that of S.vaninii.A total of 11 polyphenols was identified from S.sanghuang by ultra performance liquid chromatography tandem triple quadrupole time-of-flight mass spectrometry(UPLC-triple-TOF-MS).The components of the fermentation products of S.vaninii were relatively simple.

关 键 词：桑黄 发酵产物 DNA保护 细胞抗衰老 

分 类 号：R284[医药卫生—中药学]