不同pH下五溴联苯醚BDE-85对海水小球藻的毒性效应  

Response of Green Alga Chlorella vulgaris to Pentabromodiphenyl Ether BDE-85 Toxic Stress under Different pH Conditions

作　　者：姜乐乐 郝梦秋 顾小朵 郭晨宇 李富田 王洪斌 JIANG Lele;HAO Mengqiu;GU Xiaoduo;GUO Chenyu;LI Futian;WANG Hongbin(School of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang 222005,China)

机构地区：[1]江苏海洋大学海洋科学与水产学院,江苏连云港222005

出　　处：《水产科学》2023年第3期440-448,共9页Fisheries Science

基　　金：江苏现代农业(紫菜)产业技术体系项目(JATS2019154);江苏海洋大学大学生创新创业训练计划项目(2020035);泗洪县科技创新专项(H201925).

摘　　要：将海水小球藻培养在不同pH(8.66、8.44、8.19、7.93)下、不同含量(0、1.52、3.04、6.08、12.16、24.32μg/g)五溴联苯醚BDE-85中,在指数生长期时测定海水小球藻的比生长速率、净光合放氧速率、暗呼吸速率、叶绿素荧光参数以及超氧化物歧化酶和过氧化氢酶活性等参数,探讨不同pH条件下海水小球藻对BDE-85致毒胁迫的生理响应。试验结果表明,低pH条件下经过中、低含量BDE-85处理的海水小球藻的比生长速率呈增加趋势,其中6.08、12.16μg/g试验组较对照组分别增长了6.33%、7.39%;6.08μg/g试验组在低pH条件下净光合放氧速率比对照组高出18.43%,而24.32μg/g试验组则比对照组低39.98%;同时过氧化氢酶和超氧化物歧化酶活性增强,表明海水小球藻的抗氧化作用增强,出现了应激活性,但高含量(24.32μg/g试验组)的BDE-85处理下增强作用不明显。综上,低pH条件可以促进海水小球藻的生长,也可以缓解中、低含量的五溴联苯醚BDE-85的毒害作用,但当BDE-85含量过高时,其缓解效果受到抑制,并且在高含量的BDE-85处理下,二者的交互作用不明显。Marine green alga Chlorella vulgaris was cultured in different concentrations(0,1.52,3.04,6.08,12.16,and 24.32μg/g)of pentabromodiphenyl ether BDE-85 at different pH(8.66,8.44,8.19,and 7.93).The specific growth rate,net photosynthetic oxygen evolution rate,dark respiration rate,chlorophyll fluorescence parameters,and superoxide dismutase and catalase activities of green alga were measured during exponential growth period,to explore the toxic stress of pentabromodiphenyl ether BDE-85 on the physiological response of green alga under different pH conditions.The results showed that under low pH condition,the specific growth rate of green alga treated with low and medium mass concentration of BDE-85 was increased.The experimental groups of 6.08μg/g and 12.16μg/g increased by 6.33%and 7.39%compared with the control group,respectively.The net photosynthetic oxygen release rate of 6.08μg/g experimental group was 18.43%higher than the control group at low pH,while that of 24.32μg/g experimental group was 39.98%lower than the control group.At the same time,the activities of catalase and superoxide dismutase were increased,indicating that the antioxidant effect of green alga was enhanced,and the stress activity appeared,but the enhanced effect was not obvious under high concentration(24.32μg/g test group)BDE-85 treatment.Under low pH condition,the growth of green alga can be promoted,and the toxic effect of low and medium concentration of BDE-85 led to be alleviated.However,when the concentration of BDE-85 was too high,the toxic effect led to inhibit the growth of green alga,and the interaction between the two was not obvious under high concentration of BDE-85.

关 键 词：五溴联苯醚BDE-85 PH 小球藻 致毒胁迫 

分 类 号：S948[农业科学—水产养殖]