GA_(3)对NaCl胁迫下‘泡桐1201’幼苗生理代谢及离子吸收的影响  

Effects of GA_(3) on physiological metabolism and ion absorption of ‘Paulownia 1201’ seedlings under NaCl stress

作　　者：朱秀红[1] 杨会焕 于宏 韩钰 任方方 茹广欣[1] ZHU Xiuhong;YANG Huihuan;YU Hong;HAN Yu;REN Fangfang;RU Guangxin(College of Forestry,Henan Agricultural University,Zhengzhou 450002,China;Zhengzhou Forestry Science and Technology Demonstration Center,Zhengzhou 450000,China)

机构地区：[1]河南农业大学林学院,郑州450002 [2]郑州市林业科技示范中心,郑州450000

出　　处：《广西植物》2023年第4期688-698,共11页Guihaia

基　　金：河南省财政科技兴林项目(30602126);河南省教学名师工作室项目(21XM0413)。

摘　　要：为探究赤霉素(GA_(3))对NaCl胁迫下‘泡桐1201’幼苗生长的缓解效应及生理机制,该研究以‘泡桐1201’幼苗为材料,设置不同的NaCl、GA_(3)浓度,测定分析GA_(3)缓解NaCl胁迫下泡桐幼苗的生理指标、光合指标及离子转运能力。结果表明:(1)150 mmol·L^(-1)NaCl胁迫下泡桐幼苗的生长量显著下降(P<0.05),下降量超过50%;适宜浓度的外源GA_(3)显著提高泡桐幼苗的株高、根长及生物量,400 mg·L^(-1)的GA_(3)处理干重较A0增加69.71%。(2)随着GA_(3)浓度的增加,3种抗氧化酶(SOD、POD、CAT)活性显著提高,MDA含量显著降低,叶绿素含量、气体交换参数(P_(n)、T_(r)、C_(i)、G_(s))均增大,光合效率提高,各离子含量(K^(+)、Ca^(2+)、Mg^(2+))均呈先上升后下降的趋势;400 mg·L^(-1)的GA_(3)处理组叶片及根系中的Na^(+)含量比A0分别降低了23.59%和11.92%。(3)相关性分析及PCA分析显示,各项指标之间存在相关性,不同浓度GA_(3)处理幼苗的差异明显,当GA_(3)浓度为400 mg·L^(-1)时缓解效果最佳。综上认为,叶面喷施GA_(3)能增强盐胁迫下‘泡桐1201’幼苗的抗氧化能力,缓解因盐胁迫导致活性氧过多而引起的膜脂过氧化反应,促进植株光合作用和生物量的积累,减少植物对Na^(+)的吸收促进营养离子的积累,以添加400 mg·L^(-1)GA_(3)更能提高‘泡桐1201’幼苗的抗性。该研究结果为深入了解泡桐耐盐机理提供理论基础,也为泡桐在盐渍地开发利用提供科学依据。To explore the alleviation effect and physiological mechanism of gibberellin(GA_(3)) on the growth of ‘Paulownia 1201' seedlings under NaCl stress,this study took ‘Paulownia 1201' seedlings as materials,different NaCl and GA_(3) concentrations were set to determine and analyze the physiological indexes,photosynthetic indexes and ion transport capacity of ‘Paulownia 1201' seedlings under NaCl stress alleviated by GA_(3).The results were as follows:(1) Under 150 mmol·L^(-1) NaCl stress,the growth of ‘Paulownia 1201' seedlings decreased significantly(P0.05),which was more than 50%.The appropriate concentration of exogenous GA_(3) significantly increased the plant height,root length and biomass of ‘Paulownia 1201' seedling.The dry weight of 400 mg·L^(-1) GA_(3) treatment increased by 69.71% compared with A0.(2) With the increase of GA_(3) concentration,the activities of three antioxidant enzymes(SOD,POD,CAT) increased significantly;content of MDA decreased significantly;chlorophyll content and gas exchange parameters(P_(n)、T_(r)、C_(i)、G_(s)) were increased,and the photosynthetic efficiency increased;the contents of each ion(K^(+),Ca^(2+),Mg^(2+)) increased first and then decreased.The Na^(+) contents in leaves and roots of 400 mg·L^(-1) GA_(3) treatment group decreased by 23.59% and 11.92% compared with A0.(3) Correlation analysis and PCA analysis showed that there was a correlation between each index,seedlings which treated with different concentrations of GA_(3) had obvious differences,and the best alleviating effect was found when the concentration of GA_(3) was 400 mg·L^(-1).In summary,foliar spraying GA_(3) can enhance the antioxidant capacity of ‘Paulownia 1201' seedlings under salt stress,alleviate the membrane lipid peroxidation reaction caused by the increase of reactive oxygen species under salt stress,promote the accumulation of photosynthesis and biomass of plants,reduce the absorption of Na^(+) by plants,promote the accumulation of nutrient ions,adding 400 mg·L^(-1) GA_(3) can impr

关 键 词：‘泡桐1201’ NACL胁迫 赤霉素 离子吸收 PCA分析 

分 类 号：Q945.12[生物学—植物学]