印度南瓜高效再生体系的优化  

Optimization of High-efficiency Regeneration System in Cucurbita maxima Duch.

作　　者：疏琴 刘英[2] 邱喜岩 卢秋晨 何亚迪 孙雅佩 廖青桂 薄凯亮 段颖[1] 王长林[1] SHU Qin;LIU Ying;QIU Xiyan;LU Qiuchen;HE Yadi;SUN Yapei;LIAO Qinggui;BO Kailiang;DUAN Ying;WANG Changlin(State Key Laboratory of Vegetable Biobreeding,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Harbin Academy of Agricultural Sciences,Harbin 150030,Heilongjiang,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京100081 [2]哈尔滨市农业科学院,黑龙江哈尔滨150030

出　　处：《中国蔬菜》2023年第5期65-71,共7页China Vegetables

基　　金：北京市设施蔬菜创新团队项目(BAIC01);国家自然科学基金项目(31972410);中国农业科学院科技创新工程项目(CAASASTIP-IVFCAAS);中央级公益性科研院所基本科研业务费项目(IVF-BRF2022007)。

摘　　要：以10个基因型印度南瓜子叶节为外植体,研究了不同激素浓度配比、外植体处理方式、抑菌抗生素种类和浓度等对印度南瓜离体诱导再生的影响。结果表明:印度南瓜离体培养再生率受外源激素浓度配比和基因型的影响较大,较适宜的激素浓度配比为1.0~2.0mg·L^(-1)6-BA+0.5mg·L^(-1)ABA。外植体的切取时间、切割方式、接种方式等也是影响再生率的重要因素,播种后24~48h,切取1/2或1/4子叶节,采用斜插法接种的外植体再生率较高。外源添加100mg·L^(-1)头孢噻肟钠(Cef)能够有效抑制细菌污染。The cotyledon nodes of 10 cultivars with different genotypes were used as explants to study the effects of exogenous phytohormone concentration ratios,method of obtaining explants,types and concentrations of antibiotics on the regeneration efficiency in Cucurbita maxima Duch.The results showed that the regeneration efficiency was greatly affected by the genotype and exogenous phytohormones concentration ratio in the shoot induction medium.The optimal formula of the shoot induction medium is 1.0-2.0 mg·L^(-1)6-BA+0.5 mg·L^(-1) ABA.The cutting time,cutting method,and inoculation method of explants are also important factors affecting the regeneration efficiency.After seeding for 24-48 h,cutting 1/2 or 1/4 cotyledon nodes for explants and inserting them at 45o angle on the shoot induction medium can improve the regeneration efficiency.Supplemented with 100 mg·L^(-1) Cefotaxime sodium(Cef)in shoot induction medium can effectively inhibit bacterial infection.

关 键 词：印度南瓜 离体再生体系 子叶节 基因型 外源激素 

分 类 号：S642.1[农业科学—蔬菜学]