辽宁省韭菜灰霉病菌的分类鉴定及生物学特性研究  

Studies on Classification,Identification and Biological Characterization of Chinese Chive Gray Mold Cinerea in Liaoning Province

作　　者：陈乐 苗则彦[1] 孙柏欣[1] 赵杨[1] CHEN Le;MIAO Zeyan;SUN Baixin;ZHAO Yang(Institute of Plant Protection,Liaoning Academy of Agricultural Sciences,Shenyang 110866,Liaoning,China)

机构地区：[1]辽宁省农业科学院植物保护研究所,辽宁沈阳110161

出　　处：《中国蔬菜》2023年第5期72-78,共7页China Vegetables

基　　金：辽宁省自然基金指导计划项目(2019-ZD-0380),辽宁省农业科学院学科建设计划项目(2022DD082412)。

摘　　要：以辽宁省北镇市中安镇采集的湿腐型韭菜灰霉病菌为研究对象,采用形态学特征及分子生物学方法进行病原菌鉴定,并研究其生物学特性。结果表明,辽宁省韭菜灰霉病菌为葱鳞葡萄孢(Botrytissquamosa),菌丝生长最适碳源为葡萄糖,适宜氮源为牛肉膏、蛋白胨和酵母膏,最适生长温度为25℃,最适pH为6.0;pH为5.0~11.0及10~30℃温度条件下均能生长,超过30℃菌丝生长缓慢,35℃停止生长;菌核产生对pH和光照敏感,pH为5.0~6.0、12h/12h光暗交替培养均有利于菌核产生,pH>6.0及24h连续光照则抑制菌核产生。Taking the wet-rot type Chinese chives gray mold collected from Beizhen City of Liaoning Province as research object,this paper identified the pathogen by morphological feature and molecular biological method,and studied its biological characteristics.The results showed that the pathogen of Chinese chives gray mold was Botrytis squamosa.The optimum carbon source for mycelia growth was glucose,and suitable nitrogen source was beef extract,yeast extract and peptone.The optimum growth temperature was 25℃,and the optium pH was 6.0.The mycelium could grow under the condition of 5.0-11.0 pH and 10-30℃.Mycelium would grow slowly when the temperature was over 30℃and stop growing at 35℃.The sclerotium generation was sensitive to pH and light.The 5.0-6.0 pH,12 h/12 h alternating light and dark culture were both conductive to the generation of sclerotia.When pH was greater than 6.0 plus 24 h continuous illumination could inhibit the production of sclerotia.

关 键 词：韭菜 灰霉病菌 分类鉴定 生物学特性 

分 类 号：S436.33[农业科学—农业昆虫与害虫防治]