液质联用法同时测定化妆品中辛酰羟肟酸、对羟基苯乙酮、p-茴香酸的含量  

Simultaneous determination of caprylhydroxamic acid,4-hydroxyacetophenone and p-anisic acid in cosmetics by HPLC-MS

作　　者：李泽夏琼 邱红燕[1] 马莹[1] 刘丹 赵丽[1] Zexiaqiong Li;Hongyan Qiu;Ying Ma;Dan Liu;Li Zhao(Guizhou Institute for Food and Drug Control,Guiyang,Guizhou 550004,China)

机构地区：[1]贵州省食品药品检验所,贵州贵阳550004

出　　处：《日用化学工业（中英文）》2023年第5期596-601,共6页China Surfactant Detergent & Cosmetics

摘　　要：采用高效液相色谱-三重四极杆质谱联用仪同时检测化妆品中辛酰羟肟酸、对羟基苯乙酮、p-茴香酸的含量,以0.1%乙酸-乙腈(含0.1%甲酸)为流动相,采用梯度洗脱的方式,根据保留时间和特征离子对的相对丰度比进行定性,以特征离子的峰面积进行定量,用标准曲线法计算三种物质的含量。结果显示,水、乳、霜三种基质中辛酰羟肟酸、对羟基苯乙酮、p-茴香酸分别在10~2000,2~2000和5~2000 ng/mL时,与峰面积呈现良好的线性关系,相关系数(r)>0.999。该方法测定化妆品中辛酰羟肟酸、对羟基苯乙酮、p-茴香酸于48 h内稳定,回收率在87.37%~111.56%之间,精密度的平均相对标准偏差(RSD)分别为1.4%,1.8%和1.6%。该检测方法高效、方便且定性定量准确,适用于不同基质化妆品中辛酰羟肟酸、对羟基苯乙酮、p-茴香酸的定量定性分析。The content of caprylhydroxamic acid,4-hydroxyacetophenone and p-anisic acid in cosmetics was simultaneously determined by HPLC-MS.The mobile phase was 0.1%acetic acid in acetonitrile(containing 0.1%formic acid)by using the gradient elution method.The qualitative analysis of these components was based on the retention time and the relative abundance ratio of the characteristic ion pairs.The peak area of the characteristic ions was used for quantitative analysis.The standard curve method was used to calculate the content of the three substances.The results showed that,in liquid matrix,lotion matrix and cream matrix,caprylhydroxamic acid,4-hydroxyacetophenone and p-anisic acid showed good linear relationships in the range of 10-2000,2-2000 and 5-2000 ng/mL,respectively,while the correlation coefficients(r)were>0.999.This method was applied to determine the caprylhydroxamic acid,4-hydroxyacetophenone and p-anisic acid in cosmetics,the results of which were stable within 48 hours.The recovery rates were between 87.37%and 111.56%.The average relative standard deviation(RSD)of the precision research was 1.4%,1.8%and 1.6%,respectively.This method is efficient,convenient,and accurate in qualitative and quantitative analysis.It is suitable for quantitative and qualitative analysis of caprylhydroxamic acid,4-hydroxyacetophenone and p-anisic acid in cosmetics of different matrices.

关 键 词：液质联用法 化妆品 辛酰羟肟酸 对羟基苯乙酮 p-茴香酸 

分 类 号：TQ658[化学工程—精细化工]