HPLC法测定不同产地生何首乌和不同炮制工艺制首乌中顺(反)式二苯乙烯苷含量  

Determination of cis(trans)-THSG in Polygoni Multiflori Radix from different areas and Polygoni Multiflori Radix Preparata using different processing methods by UPLC

作　　者：王雪婷 杨建波[1] 高慧宇 宋云飞 程显隆[1] 辜冬琳 王莹[1] 马双成[1] 魏锋[1] WANG Xueting;YANG Jianbo;GAO Huiyu;SONG Yunfei;CHENG Xianlong;GU Donglin;WANG Ying;MA Shuang-cheng;WEI Feng(National Institutes for Food and Drug Control,Beijing 100050,China)

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《中国药物警戒》2023年第4期383-387,共5页Chinese Journal of Pharmacovigilance

基　　金：国家重点研发计划(2019YFC1711500);国家自然科学基金资助项目(81973476、81773874)。

摘　　要：目的比较不同产地何首乌及不同炮制工艺制何首乌中顺(反)二苯乙烯苷含量的变化,探究其炮制减毒增效的作用机制,为何首乌质量控制及饮片炮制标准研究提供数据参考,以期降低临床用药风险。方法采用高效液相色谱法,测定何首乌和制何首乌中顺(反)式二苯乙烯苷(2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷)的含量。结果不同批次何首乌和制何首乌中顺(反)式二苯乙烯苷含量范围分别为27.23~358.19μg·g^(-1)和10.30~52.00 mg·g^(-1)。结论本方法操作简单,准确度高,重复性好,可以用于何首乌和制何首乌中顺(反)式二苯乙烯苷含量测定。Objective To provide data for quality control of Polygoni Multiflori Radix(PMR)and processing standards for Polygoni Multiflori Radix Preparata(PMRP)by observing the changes of cis(trans)-THSG(2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside)of this plant grown in different areas and processed differently and to explore the mechanism by which processing can reduce its toxicity and increase efficacy.Methods An HPLC method was employed to determine the contents of cis(trans)-THSG.Results The content of cis(trans)-THSG in PMR and PMRP ranged from 27.23 to 358.19μg·g^(-1) and from 10.30 to 52.00 mg·g^(-1) respectively.Conclusion This method is simple,accurate and reproducible,which can be used for content determination of cis(trans)-THSG in PMR and PMRP.

关 键 词：何首乌 制何首乌 高效液相色谱法 顺式二苯乙烯苷 反式二苯乙烯苷 

分 类 号：R283[医药卫生—中药学] R994.11[医药卫生—中医学]