唾液乳杆菌在类风湿性关节炎疾病进展中的作用  

Role of Lactobacillus salivary in the Progression of Rheumatoid Arthritis

作　　者：袁飞 黄暖琴[1,2] 黄莹 萧韵健[1,2] 李丽[1,2] 杨彩红 YUAN Fei;HUANG Nuanqin;HUANG Ying;XIAO Yunjian;LI Li;YANG Caihong(Dongguan Hospital Affiliated to Southern Medical University;Dongguan People’s Hospital,Dongguan 523058,China)

机构地区：[1]南方医科大学附属东莞医院 [2]东莞市人民医院,广东东莞523058

出　　处：《北华大学学报（自然科学版）》2023年第3期326-331,共6页Journal of Beihua University（Natural Science）

基　　金：东莞市社会科技发展重点项目(202050715001204).

摘　　要：目的探讨唾液乳杆菌在类风湿关节炎(rheumatoid arthritis,RA)发病中的作用及其机制.方法选取32只SPF级雄性SD大鼠,将大鼠分为健康对照组、CIA模型组(胶原诱导型(collagen-induced arthritis,CIA)大鼠模型)、唾液乳杆菌干预组、甲氨蝶呤组,每组各8只;观察大鼠外观形态,行CIA评分、足跖容积测量及HE染色,应用宏基因检测乳杆菌种类及丰富度.结果与模型组相比,唾液乳杆菌干预可明显改善RA大鼠组织病理状态,减低关节炎严重程度,改善关节肿胀;与模型组相比,唾液乳杆菌干预组和甲氨蝶呤组的相似度、丰富度(S)、均匀度(E)和Shannon-Wiener指数(H)均显著升高.结论唾液乳杆菌对类风湿性关节炎可能有一定的治疗效果和预防作用,可为类风湿性关节炎的治疗提供新思路.Objective To investigate the role and mechanism of Lactobacillus salivary in the pathogenesis of rheu-matoid arthritis.Method A collagen-induced arthritis(CIA)rat model was established in 32 male SD rats raised in an SPF level animal laboratory.The experiment was divided into four groups(healthy control group,CIA model group,Lactobacillus salivary intervention group,and methotrexate group).The appearance,CIA score,plantar volume measurement,HE staining,and macrogene detection of Lactobacillus species and abundance were perfo-rmed.Results Compared with the model group,the intervention with Lactobacillus salivary can significantly improve the histopathological state of RA rats,reduce the severity of arthritis,and improve joint swelling.Compared with the model group,the similarity,abundance(S),uniformity(E),and Shannon Wiener index(H)of Lactob-acillus salivary intervention group and the methotrexate group were significantly increased.Conclusion Lactob-acillus salivary has significant therapeutic effects on rheumatoid arthritis model rats,which may provide a new idea for the clinical treatment of rheumatoid arthritis.

关 键 词：大鼠 类风湿性关节炎 唾液乳杆菌 

分 类 号：R593.22[医药卫生—内科学]