一株黄颡鱼源维氏气单胞菌的分离鉴定及其对10种中草药的敏感性研究  

Isolation and identification of a strain of Aeromonas veronii from Pelteobagms fulvidraco and drug susceptibility to 10 kinds of Chinese herbal medicines

作　　者：张平香 魏朝辉 郑程鹏[1,2] 张浔 张棋 蒋佳辉 罗杨志 喻运珍[1,2] 艾桃山 张立强[1,2] 朱文欢 黄天乐[5] 邓平 ZHANG Pingxiang;WEI Chaohui;ZHENG Chengpeng;ZHANG Xun;ZHANG Qi;JIANG Jiahui;LUO Yangzhi;YU Yunzhen;AI Taoshan;ZHANG Liqiang;ZHU Wenhuan;HUANG Tianle;DENG Ping(Wuhan Academy of Agricultural Sciences,Wuhan 430207,China;Wuhan Chopper Fishery Bio-Tech Co.,Ltd.,Wuhan 430070,China;College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China;Honghu Fishery Development Center,Honghu 433200,China;Fisheries Technical Extension and Guidance Center of Wuhan,Wuhan 430014,China)

机构地区：[1]武汉市农业科学院,武汉430207 [2]武汉中博水产生物技术有限公司,武汉430070 [3]华中农业大学水产学院,武汉430070 [4]洪湖市水产发展中心,湖北洪湖433200 [5]武汉市水产技术推广指导中心,武汉430014

出　　处：《水产科技情报》2023年第3期176-180,共5页Fisheries Science & Technology Information

基　　金：湖北省技术创新专项(2018ABA101);国家特色淡水鱼产业技术体系(CARS-46)。

摘　　要：从湖北省某养殖场患病黄颡鱼组织中分离到1株细菌性病原,经形态学观察、16S rRNA基因序列测定及系统发育学分析,鉴定为维氏气单胞菌(Aeromonas veronii)。为确定10种中草药(诃子、五倍子、穿心莲、乌梅、连翘、五味子、秦皮、鱼腥草、牡丹皮、青蒿)对黄颡鱼源维氏气单胞菌的抑菌效果,分别制备这10种中草药的水提物,使用不同浓度梯度的水提物进行了抑菌试验,并以盐酸多西环素和氟苯尼考作阳性对照。试验结果表明:诃子、五倍子、穿心莲有较好的抑菌效果,其中诃子抑菌效果最好;乌梅、连翘、五味子、秦皮、鱼腥草、牡丹皮、青蒿的抑菌效果较差,其中五味子的抑菌效果最差。A bacterial pathogen isolated from the tissue of Pelteobagms fulvidraco was identified as Aeromonas veronii by morphological observation,16S rRNA gene sequence and phylogenetic analysis.In order to determine the bacteriostatic effect of 10 Chinese herbs(chebulae fructus,gallnut,paniculate,dark plum,fructus forsythia,fructus schisandra,cortex fraxini,Houttuynia cordata,moutan bark,artemisia annul)against aeromonas virginiae of P.fulvidraco,water extracts of 10 herbs with different concentration gradients were prepared.Doxycycline hydrochloride and florfenicol were used as positive controls.The experiment result showed that chebulae fructus,gallnut and paniculata had preferable bacteriostatic effect,and chebulae fructus had the best bacteriostatic effect.The antibacterial effect of dark plum,fructus forsythiae,fructus schisandra,cortex fraxini,Houttuynia cordata,moutan bark and artemisia annul was poor,and the antibacterial effect of fructus schisandra was the worst.

关 键 词：黄颡鱼 维氏气单胞菌 16S rRNA 中草药 抑菌试验 

分 类 号：S943[农业科学—水产养殖]