套种绿肥对尾巨桉人工林土壤细菌群落特征的影响  

Effects of Green Manure Intercropping on Soil Bacterial Community of Eucalyptus urophylla×Eucalyptus grandis Plantation

作　　者：朱孔鑫 庄舜尧[2] 钱壮壮 王会利[3] 王艮梅[1] ZHU Kongxin;ZHUANG Shunyao;QIAN Zhuangzhuang;WANG Huili;WANG Genmei(Co-Innovation Center for the Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,China;Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008,China;Academy of Forestry Research of Guangxi Zhuang Autonomous Region,Nanning 530002,China)

机构地区：[1]南京林业大学南方现代林业协同创新中心,南京210037 [2]中国科学院南京土壤研究所,南京210008 [3]广西壮族自治区林业科学研究院,南宁530002

出　　处：《土壤》2023年第2期313-320,共8页Soils

基　　金：广西创新驱动发展专项资金项目(桂科AA17204087-11)资助。

摘　　要：不合理的桉树人工林种植会对土壤质量产生负面影响。本研究通过田间试验,探讨不同豆科绿肥套种对土壤养分、酶活性以及细菌群落特征的影响,分析在桉树人工林套种绿肥的可行性,从而为改善桉树人工林土壤质量提供参考。试验设置白灰毛豆套种、田菁套种以及无套种对照3种处理。结果表明:套种白灰毛豆和套种田菁可提高土壤养分含量、微生物生物量及酶活性;两种绿肥套种措施对土壤细菌多样性无显著影响,而细菌群落结构存在较大差异;套种田菁时厚壁菌门、绿弯菌门、产醋杆菌属、瘤胃梭菌属和Candidatus Solibacter属的相对丰度较高,而套种白灰毛豆时变形菌门和铁还原菌属的相对丰度更高;细菌群落功能预测表明,套种白灰毛豆的固氮和硝酸盐还原功能显著表达,而套种田菁的芳香族化合物降解功能显著表达。相关性分析表明,土壤有效硼是微生物生物量及酶活性的主要影响因子,而水解氮是细菌门和属相对丰度的主要影响因子。综上,林下套种豆科绿肥可以改善土壤养分状况,提高土壤酶活性,调节土壤微生物群落结构,可作为改善桉树人工林土壤质量和实现桉树人工林可持续经营的有效措施。Irrational Eucalyptus plantation could induce the degradation of soil quality.A field experiment was conducted to investigate the effects of green manure intercropping on soil nutrient,enzyme activity and bacterial community in Eucalyptus urophylla×Eucalyptus grandis plantation in order to guide Eucalyptus sustainable production,in which three treatments of Tephrosia candida(TC),Sesbania cannabina(SC)and control without green manure(CK)were setup.The results show that both TC and SC increase soil available nutrients,enzyme activity and microbial biomass.The relative abundances of Firmicutes,Chloroflexi,Oxobacter,Ruminiclostridium and Candidatus Solibacter are higher under SC,while those of Proteobacteria and Thermincola are higher under TC.TC greatly influences nitrogen fixation and nitrate reduction related bacteria,while SC significantly affects aromatic compound degradation related bacteria.Soil available boron is the main factor influencing soil microbial mass and enzyme activity,while soil alkaline hydrolysis nitrogen is the main factor affecting the abundances of bacteria in phylum and family levels.In conclusion,green manure intercropping can improve soil nutrient,increase soil enzyme activity and regulate soil microbial structure,thus it is an effective measure for Eucalyptus sustainable production.

关 键 词：桉树 绿肥 土壤养分 细菌群落 酶活性 

分 类 号：S723.8[农业科学—林木遗传育种]