GK糖尿病大鼠感染创面的生物学特性研究  

Biological Characteristics of Infected Wounds in GK Diabetes Rats

作　　者：金达康 谢仕源 梁瑶 王柳萍 杨斌[1] JIN Da-kang;XIE Shi-yuan;LIANG Yao;WANG Liu-ping;YANG Bin(College of Pharmacy,Guangxi Medical University,Nanning,Guangxi Autonomous Region,530021;College of Pharmacy,Guangxi University of Traditional Chinese Medicine,Nanning,Guangxi Autonomous Region)

机构地区：[1]广西医科大学药学院,530021 [2]广西中医药大学药学院

出　　处：《岭南急诊医学杂志》2023年第2期95-97,111,共4页Lingnan Journal of Emergency Medicine

基　　金：广西中医药管理局科研课题(GXZYZ20210068)。

摘　　要：目的:建立GK大鼠全皮层切除合并感染模型,探讨其生物学特性改变,为GK大鼠应用于糖尿病足感染创面的治疗研究提供参考依据。方法:取SPF级的雄性GK大鼠和雄性Wistar大鼠,建立感染创面模型,于不同时间点对创面进行拍照后计算创面愈合率。在D1、D3、D7、D14和D21,对换下敷料进行细菌培养计数。在第D22,取大鼠清血和创面组织,用ELISA法测定血清中降钙素原(PCT)和超敏C⁃反应蛋白(hs⁃CRP)的含量,创面组织行HE染色观察组织病理学情况。结果:相对于Wistar大鼠,在D3、D7、D11、D14、D17时间点,GK大鼠的创面愈合率显著减少;在D7时间点,GK大鼠感染创面的纱布敷料菌落数显著增加。另外,GK大鼠血清中PCT、hs⁃CRP均显著增加。HE染色结果显示,GK大鼠创面的新生表皮较薄。结论:GK糖尿病大鼠感染创面是一种适用于糖尿病足感染治疗相关研究的动物模型。Objective:To establish an animal model of GK rats with total cortical excision and infection,and ex⁃plore the changes of its biological characteristics,so as to provide a reference for GK rats to be used in the treatment of diabetes foot infection.Methods:Take SPF grade male GK rats and male Wistar rats to establish infected wound mod⁃els,take photos of the wound at different time points and calculate the wound healing rate.At D1,D3,D7,D14 and D21,the dressings were taken for bacterial culture count.At D22,the blood and wound tissues of rats were taken,and the contents of procalcitonin(PCT)and hypersensitive C⁃reactive protein(hs⁃CRP)in serum were determined by ELI⁃SA.The wound tissues were stained with HE to observe the histopathological conditions.Results:Compared with Wistar rats,the wound healing rate of GK rats decreased significantly at time points D3,D7,D11,D14 and D17;At the D7 time point,the number of colonies of gauze dressing on the infected wound of GK rats increased significantly.In addi⁃tion,PCT and hs⁃CRP in serum of GK rats increased significantly.HE staining results showed that the new epidermis of the wound surface of GK rats was thinner.Conclusion:The infected wound of GK diabetes rats is an animal model suit⁃able for the research related to the treatment of diabetes foot infection.

关 键 词：GK大鼠 糖尿病 感染创面 生物学特性 

分 类 号：R587.2[医药卫生—内分泌]