军用密闭舱室噪声及CO联合染毒致大鼠生殖损伤效应  

Combined exposure to noise and CO induces adverse male reproductive effects in rats

作　　者：李迎庆 刘畅[1] 张中豪 王建康 孙磊[1] 韩飞[1] 曹佳[1] 刘晋祎[1] LI Yingqing;LIU Chang;ZHANG Zhonghao;WANG Jiankang;SUN Lei;HAN Fei;CAO Jia;LIU Jinyi(Institute of Toxicology,Faculty of Military Preventive Medicine,Army Medical University(Third Military Medical University),Chongqing,400038,China)

机构地区：[1]陆军军医大学(第三军医大学)军事预防医学系毒理学研究所,重庆400038

出　　处：《陆军军医大学学报》2023年第9期911-919,共9页Journal of Army Medical University

基　　金：军队计划生育科研课题(16JS008);国家自然科学基金重点项目(82130097)。

摘　　要：目的探讨密闭舱室噪声、一氧化碳(carbon monoxide,CO)单独和联合染毒对雄性大鼠生殖系统的损伤效应,评价二者是否具有联合效应以及损伤可能涉及的信号通路。方法50只雄性SD大鼠,采取随机数字表法分为空白对照组、阴性对照组(单纯束缚,不染毒)、噪声染毒组(噪声强度为85 dB)、CO染毒组(CO浓度为460 mg/m3)、联合染毒组(CO浓度460 mg/m3、噪声强度85 dB),每组10只。大鼠固定于口鼻染毒杯并放置于联合模拟染毒舱中,按照染毒流程每天染毒2 h,连续30 d。染毒结束后分析精子质量,包括精子运动能力、精子密度等,通过TUNEL染色确定睾丸细胞凋亡水平。睾丸组织送样进行circRNA测序,测序数据行生物信息学富集分析。结果与阴性对照组相比,噪声染毒组和联合染毒组精液密度均显著降低(P<0.05)。在精子运动能力方面,与阴性对照组相比,CO组显著下降(P<0.05),联合染毒组精子运动能力下降更为显著(P<0.05)。噪声染毒组、CO染毒组大鼠睾丸组织可见少量空泡形成,联合染毒组大鼠睾丸组织多处可见空泡形成,生精小管管腔内细胞排列紊乱。噪声染毒组和CO染毒组细胞凋亡增多,联合染毒组细胞凋亡数目最多。测序数据生物信息学分析结果提示,关键应答circRNA及其调控的关键分子可能参与噪声和CO染毒对大鼠睾丸的损伤效应,包括Smc4、Sycp1、Sycp3、Spag17、Mns1、Top2a和Rock1等,主要参与减数分裂、精子形成、细胞周期及凋亡通路。结论噪声和CO可致雄性生殖损伤并存在典型的联合作用,且生殖损伤与circRNAs调控相关。Objective To explore the male reproductive toxicity in rats caused by single or combined exposure to noise and carbon monoxide(CO)in closed cabin,and evaluate the joint effects and associated signal pathways.Methods Fifty male SD rats were randomly divided into blank control group,restraint group(negative control group),noise exposure group,CO exposure group and combined group(n=10).According to the poisoning procedure,the rats were fixed in the oral and nasal toxic cup and placed in the chamber and they were poisoned for 2 h per day for 30 consecutive days.The sperm quality,including sperm motility and sperm density,was analyzed after exposure,and the apoptosis of testicular cells was determined by TUNEL staining.The testicular tissues were harvested for circRNA sequencing,and the sequencing data were analyzed by bioinformatics enrichment.Results After exposure to noise and CO,the semen density of the noise group and combine group was decreased significantly(P<0.05).The sperm motility was obviously lower in the CO group than the negative control group(P<0.05),and more serious decline was observed in the combined group(P<0.05).Morphological study showed that there were a few vacuoles in the testis of rats exposed to noise or CO and much more vacuoles in the testis of rats exposed to combination.Obvious pathological damage was found in the testis of rats combined exposed to noise and CO,and the cells in the seminiferous tubules were in a disordered arrangement.The number of apoptotic cells was increased in the noise and CO group,and the number was the largest in the combined group.The results of sequencing analysis suggested that key circRNA and their regulated key molecules might be involved in the reproductive damage effects of noise and CO exposure on rat testes,including Smc4,Sycp1,Sycp3,Spag17,Mns1,Top2a and Rock1,which played important roles in meiosis,spermatogenesis,cell cycle and apoptosis pathways.Conclusion Combined exposure to noise and CO shows typical joint effect on male reproductive damages,which a

关 键 词：噪声 一氧化碳 联合染毒 生殖系统损伤 细胞凋亡 circRNA 

分 类 号：R136[医药卫生—劳动卫生] R698.2[医药卫生—公共卫生与预防医学] R821.2