城市污水中诺如病毒两种富集浓缩方法的比较研究  

Two enrichment methods for norovirus in urban sewage:a comparative study

作　　者：侯敏 丁珵[1] 邓富昌 田哲[2,3] 丁培[1] 王友斌[1] 徐凯强 姚巧 唐宋 姚孝元[1] HOU Min;DING Cheng;DENG Fu-chang;TIAN Zhe;DING Pei;WANG You-bin;XU Kai-qiang;YAO Qiao;TANG Song;YAO Xiao-yuan(China CDC Key Laboratory of Environment and Population Health/National Institute of Environmental Health,Chinese Center for Disease Control and Prevention,Beijing 100021,China;Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences/State Key Laboratory of Environmental Aquatic Chemistry;Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences/National Engineering Research Center of Industrial Wastewater Detoxication and Resource Recovery;Center for Global Health,School of Public Health,Nanjing Medical University)

机构地区：[1]中国疾病预防控制中心环境与人群健康重点实验室、中国疾病预防控制中心环境与健康相关产品安全所,北京100021 [2]中国科学院生态环境研究中心环境水质学国家重点实验室 [3]中国科学院生态环境研究中心工业废水无害化与资源化国家工程研究中心 [4]南京医科大学公共卫生学院全球健康中心

出　　处：《环境卫生学杂志》2023年第5期357-362,共6页JOURNAL OF ENVIRONMENTAL HYGIENE

基　　金：首都卫生发展科研专项重点攻关项目(首发2022-1G-4231);国家重点研发计划(2022YFC3204700)。

摘　　要：目的比较聚乙二醇沉淀法和阴离子膜吸附—超声振荡法富集浓缩污水中诺如病毒(norovirus,NoV)的回收率,选择更适用于城市污水中诺如病毒监测工作的富集浓缩方法。方法将诺如病毒稀释三个梯度分别加入到城市生活污水(100 mL)中,采用聚乙二醇沉淀法和阴离子膜吸附—超声振荡法进行富集浓缩,采用实时荧光反转录聚合酶链式反应(RT-PCR)检测富集浓缩后GI型和GⅡ型病毒浓度,并进一步计算回收率。结果结果表明:①当NoV GI型浓度为10^(1.4) copies/μL时,阴离子膜吸附—超声振荡法回收率高于聚乙二醇沉淀法(P<0.05)。②当NoV GⅡ型浓度为10^(2.6) copies/μL时,阴离子膜吸附—超声振荡法回收率高于聚乙二醇沉淀法(P<0.05)。结论综合考虑富集浓缩效率等相关因素,阴离子膜吸附—超声振荡法更适合小体积污水中诺如病毒的富集浓缩。Objective To investigate a more suitable enrichment method for norovirus(NoV)monitoring in urban sewage by comparing the recovery rate of NoV in sewage by polyethylene glycol(PEG)precipitation versus electronegative membrane adsorptionultrasonic oscillation.Methods NoV was diluted in three gradients and added into urban domestic sewage(100 mL),then concentrated by the two method of PEG precipitation and electronegative membrane adsorption-ultrasonic oscillation.The concentrations of GI and GⅡvirus after enrichment were detected by real-time fluorescent reverse transcription polymerase chain reaction(RT-PCR),and then the recovery rate was calculated.Results When the concentration of NoV GI was 10^(1.4) copies/μL,electronegative membrane adsorption-ultrasonic oscillation had a higher recovery rate than PEG precipitation(P<0.05).When the concentration of NoV GII was 10^(2.6) copies/μL,electronegative membrane adsorption-ultrasonic oscillation had a higher recovery rate than PEG precipitation(P<0.05).Conclusion Considering concentration efficiency and other related factors,the method of electronegative membrane adsorption-ultrasonic oscillation is more suitable for the enrichment of NoV in a small volume of sewage.

关 键 词：诺如病毒 PEG沉淀法 富集浓缩 阴离子膜 城市污水 

分 类 号：R115[医药卫生—公共卫生与预防医学] R184.1