孔雀球虫的分子鉴定及其ITS rRNA基因序列分析  

Molecular Identification and Gene Sequence Analysis Based on ITS rRNA Genes of Coccidia in Peacocks

作　　者：张誉方 刘永张[2] 贺君君 邹丰才 杨建发 ZHANG Yu-fang;LIU Yong-zhang;HE Jun-jun;ZOU Feng-cai;YANG Jian-fa(College of Veterinary Medicine,Yunnan Agricultural University,Kunming,Yunnan,650201,China;Kunming Zoo,Kunming,Yunnan,650021,China)

机构地区：[1]云南农业大学动物医学院,云南昆明650201 [2]云南省昆明动物园,云南昆明650021

出　　处：《动物医学进展》2023年第6期139-142,共4页Progress In Veterinary Medicine

基　　金：云南省朱兴全专家工作站项目(202005AF150041);云南农业大学兽医公共卫生省创新团队项目(202105AE160014)。

摘　　要：为了解云南省昆明市孔雀感染球虫的种类及种属关系,采集昆明市2个动物园孔雀粪便样品及肠道内容物样本进行球虫卵囊的收集,进行形态学鉴定,PCR扩增球虫ITS rRNA基因,测序和序列分析,并利用MEGA6.0软件构建遗传系统进化树。结果显示,扩增的ITS rRNA基因序列长度为492 bp和493 bp,共鉴定出2种球虫,分别为和缓艾美耳球虫(Eimeria mitis)和火鸡和缓艾美耳球虫(E.meleagrimitis),相似度均为100%;进化分析结果表明,孔雀和火鸡之间可能存在球虫的交叉感染风险。该研究结果丰富了野生雉类寄生虫病的资料。To understand the species and the genus relationship of coccidia from peacocks in Kunming,Yunnan province,we collected the peacock fecal samples and intestinal content samples to collect the coccidia,and performed morphological identification,PCR amplified ITS rRNA gene sequence,sequencing analysis,and constructed the phylogenetic tree using MEGA6.0 software.The results showed that the amplified ITS rRNA genes were 492 bp and 493 bp in length,and a total of two species coccidia were detected,including Eimeria mitis and E.meleagrimitis,and the similarity is up to 100%;and the evolutionary analysis showed that there may be a risk of cross-infection of the coccidia between peacock and turkey.The research results enrich the parasitic disease research of wild pheasants.

关 键 词：孔雀 球虫 ITS基因 基因序列分析 

分 类 号：S852.723[农业科学—基础兽医学] S858.39[农业科学—兽医学]